Ser12

Site Information
rLQKrLAssVLRCGK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4272220
Available spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 5 , 6 ) , mass spectrometry (in vitro) ( 4 )
Disease tissue studied:	lung cancer ( 2 , 3 ) , non-small cell lung cancer ( 2 , 3 ) , non-small cell lung adenocarcinoma ( 2 , 3 ) , neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 2 ) , Cal-12T (pulmonary) ( 3 ) , HCC44 (pulmonary) ( 3 ) , HeLa (cervical) ( 4 ) , Jurkat (T lymphocyte) ( 5 , 6 ) , LAN-6 (neural crest) ( 1 ) , lung ( 2 , 3 ) , NCI-H1355 (pulmonary) ( 2 ) , NCI-H1437 (pulmonary) ( 3 ) , NCI-H1666 (pulmonary) ( 3 ) , NCI-H1792 (pulmonary) ( 2 ) , NCI-H23 (pulmonary) ( 2 ) , NCI-H2405 (pulmonary) ( 3 ) , NCI-H441 (pulmonary) ( 2 ) , SH-SY5Y (neural crest) ( 1 ) , SMS-KCN-A (neural crest) ( 1 )

Upstream Regulation
Kinases, in vitro:	Chk1 (human) ( 4 )

References
1	Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130 25884760 Curated Info
2	Rikova K, Hall B (2013) CST Curation Set: 20736, 21163, 30158, 30159, 30160; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
3	Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
4	Blasius M, et al. (2011) A phospho-proteomic screen identifies substrates of the checkpoint kinase Chk1. Genome Biol 12, R78 21851590 Curated Info
5	Moritz A (2009) CST Curation Set: 8726; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
6	Possemato A (2009) CST Curation Set: 8361; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986 Curated Info