Thr509
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Home > Phosphorylation Site Page: > Thr509  -  VCP (human)

Site Information
kFLkFGMtPskGVLF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4271173

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 2 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 2 )
Disease tissue studied:
breast cancer ( 4 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 , 4 ) , cervical cancer ( 10 ) , cervical adenocarcinoma ( 10 )
Relevant cell line - cell type - tissue:
breast ( 3 , 4 ) , HEK293T (epithelial) ( 11 ) , HeLa (cervical) ( 6 ) , HeLa S3 (cervical) ( 10 , 12 ) , HeLa_Meta (cervical) ( 9 ) , HeLa_Pro (cervical) ( 9 ) , HeLa_Telo (cervical) ( 9 ) , Jurkat (T lymphocyte) ( 7 , 8 ) , K562 (erythroid) ( 6 ) , Vero E6-S ('epithelial, kidney') ( 1 )

Upstream Regulation
Kinases, in vitro:
CDK4 (human) ( 2 )

Downstream Regulation
Effects of modification on VCP:
intracellular localization ( 2 ) , molecular association, regulation ( 2 ) , phosphorylation ( 2 )
Effects of modification on biological processes:
cell cycle regulation ( 2 )
Induce interaction with:
CLN3 (human) ( 2 ) , OTUB1 (human) ( 2 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182
32645325   Curated Info

2

Parisi E, Yahya G, Flores A, Aldea M (2018) Cdc48/p97 segregase is modulated by cyclin-dependent kinase to determine cyclin fate during G1 progression. EMBO J
29950310   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Guo A (2011) CST Curation Set: 12435; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

8

Guo A (2011) CST Curation Set: 12436; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

9

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

10

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

11

Mori-Konya C, et al. (2009) p97/valosin-containing protein (VCP) is highly modulated by phosphorylation and acetylation. Genes Cells 14, 483-97
19335618   Curated Info

12

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

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