Ser8

Site Information
MAMssGGsGGGVPEQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4269865

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ) , mass spectrometry (in vitro) ( 9 ) , mutation of modification site ( 9 )
Disease tissue studied:	leukemia ( 8 ) , acute myelogenous leukemia ( 8 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 )
Relevant cell line - cell type - tissue:	293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 11 ) , A498 (renal) ( 13 ) , Calu 6 (pulmonary) ( 5 ) , COS7 (fibroblast) ( 9 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 6 ) , Flp-In T-Rex-293 (epithelial) ( 6 ) , H2009 (pulmonary) ( 5 ) , H2077 (pulmonary) ( 5 ) , H2887 (pulmonary) ( 5 ) , H322M (pulmonary) ( 5 ) , HCC1359 (pulmonary) ( 5 ) , HCC2279 (pulmonary) ( 5 ) , HCC366 (pulmonary) ( 5 ) , HCC4006 (pulmonary) ( 5 ) , HCC78 (pulmonary) ( 5 ) , HeLa (cervical) ( 2 , 14 ) , HeLa S3 (cervical) ( 15 , 17 ) , HeLa_Meta (cervical) ( 12 ) , HeLa_Pro (cervical) ( 12 ) , HeLa_Telo (cervical) ( 12 ) , HUES-9 ('stem, embryonic') ( 10 ) , Jurkat (T lymphocyte) ( 3 ) , K562 (erythroid) ( 4 , 14 ) , KG-1 (myeloid) ( 8 ) , LOU-NH91 (squamous) ( 5 ) , MV4-11 (macrophage) ( 16 ) , NCI-H1568 (pulmonary) ( 5 ) , NCI-H1648 (pulmonary) ( 5 ) , NCI-H2172 (pulmonary) ( 5 ) , NCI-H460 (pulmonary) ( 5 ) , NCI-H647 (pulmonary) ( 5 )

Upstream Regulation
Putative in vivo kinases:	PKACA (human) ( 9 )
Kinases, in vitro:	PKACA (human) ( 9 )
Treatments:	KT5720 ( 9 ) , MG132_withdrawal ( 12 ) , NKH_477 ( 9 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
3	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
4	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
5	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
6	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
7	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
8	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
9	Wu CY, et al. (2011) Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A. Biochim Biophys Acta 1814, 1134-9 21609790 Curated Info
10	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
11	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
12	Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81 20097925 Curated Info
13	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
14	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
15	Malik R, et al. (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 8, 4553-63 19691289 Curated Info
16	Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64 19369195 Curated Info
17	Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48 18691976 Curated Info