Ser272

Site Information
ELDELMAsLSDFKFM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 465466

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 3 , 4 ) , phospho-antibody ( 1 , 5 , 6 ) , western blotting ( 1 , 5 )
Disease tissue studied:	prostate cancer ( 1 )
Relevant cell line - cell type - tissue:	DU 145 (prostate cell) ( 1 ) , endothelial-pulmonary artery ( 5 ) , HeLa (cervical) ( 3 ) , HPAEC (endothelial) ( 4 ) , WM115 (melanocyte) ( 2 )

Downstream Regulation
Effects of modification on PXN iso2:	intracellular localization ( 3 , 6 ) , molecular association, regulation ( 3 , 6 )
Effects of modification on biological processes:	cell adhesion, altered ( 1 , 4 , 6 ) , cell motility, altered ( 6 ) , cytoskeletal reorganization ( 4 , 5 )
Induce interaction with:	ARHGEF6 (human) ( 6 ) , GIT1 (human) ( 6 )
Inhibit interaction with:	GIT1 (human) ( 3 )

References
1	Wells CM, et al. (2010) PAK4: a pluripotent kinase that regulates prostate cancer cell adhesion. J Cell Sci 123, 1663-73 20406887 Curated Info
2	Old WM, et al. (2009) Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Mol Cell 34, 115-31 19362540 Curated Info
3	Dong JM, et al. (2009) Paxillin nuclear-cytoplasmic localization is regulated by phosphorylation of the LD4 motif: evidence that nuclear paxillin promotes cell proliferation. Biochem J 418, 173-84 18986306 Curated Info
4	Birukova AA, Cokic I, Moldobaeva N, Birukov KG (2009) Paxillin is involved in the differential regulation of endothelial barrier by HGF and VEGF. Am J Respir Cell Mol Biol 40, 99-107 18664639 Curated Info
5	Birukova AA, et al. (2008) Cross talk between paxillin and Rac is critical for mediation of barrier-protective effects by oxidized phospholipids. Am J Physiol Lung Cell Mol Physiol 295, L593-602 18676874 Curated Info
6	Nayal A, et al. (2006) Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. J Cell Biol 173, 587-9 16717130 Curated Info