Ser257
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Home > Phosphorylation Site Page: > Ser257  -  GJA1 (rat)

Site Information
TTGPLsPskDCGsPk   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 465456

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 ) , mass spectrometry (in vitro) ( 3 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Relevant cell line - cell type - tissue:
heart ( 2 , 4 ) , IEC-6 (epithelial) ( 1 ) , myometrial ( 5 )

Upstream Regulation
Kinases, in vitro:
CAMK2A (human) ( 3 )
Treatments:
2,2'-DCB ( 5 ) , PD184352 ( 1 ) , PD98059 ( 5 ) , serum ( 1 )

Downstream Regulation
Effects of modification on GJA1:
activity, inhibited ( 5 )

References 

1

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Huang RY, et al. (2011) Identification of CaMKII phosphorylation sites in Connexin43 by high-resolution mass spectrometry. J Proteome Res 10, 1098-109
21158428   Curated Info

4

Axelsen LN, et al. (2006) Identification of ischemia-regulated phosphorylation sites in connexin43: A possible target for the antiarrhythmic peptide analogue rotigaptide (ZP123). J Mol Cell Cardiol 40, 790-8
16678851   Curated Info

5

Chung D, Loch Caruso R (2005) 2,2'-Dichlorobiphenyl decreases amplitude and synchronization of uterine contractions through MAPK1-mediated phosphorylation of GJA1 (connexin43) and inhibition of myometrial gap junctions. Biol Reprod 73, 974-82
16000550   Curated Info