Ser255
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Home > Phosphorylation Site Page: > Ser255  -  MKK4 (mouse)

Site Information
ISGQLVDsIAKtRDA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447654

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , phospho-antibody ( 13 , 14 , 15 ) , western blotting ( 14 , 15 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 11 ) , 'brain, cerebral cortex' ( 11 ) , 'brain, hippocampus, dentate gyrus' ( 11 ) , 'brain, midbrain' ( 11 ) , 'brain, striatum' ( 15 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , brain ( 6 , 7 , 9 , 10 ) , embryo ( 7 ) , FL5.12 (lymphoid) ( 14 ) , heart ( 9 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 3 ) , kidney ( 9 ) , liver ( 7 ) , lung ( 9 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) [IGF1R (mouse)] ( 13 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 8 ) , pancreas ( 9 )

Upstream Regulation
Regulatory protein:
Cot (mouse) ( 13 ) , IGF1R (mouse) ( 14 ) , NMNAT1 (mouse) ( 2 ) , SARM1 (mouse) ( 2 )
Kinases, in vitro:
DLK (mouse) ( 2 ) , MLK3 (human) ( 16 )
Treatments:
axatomy ( 2 ) , IGF-1 ( 14 ) , ipatasertib ( 2 ) , metamfetamine ( 15 ) , MK-2206 ( 2 ) , nerve_damage ( 2 ) , TNF ( 13 )

References 

1

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

2

Yang J, et al. (2015) Pathological axonal death through a MAPK cascade that triggers a local energy deficit. Cell 160, 161-76
25594179   Curated Info

3

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

6

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

7

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

8

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

11

Trinidad JC, et al. (2008) Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7, 684-96
18056256   Curated Info

12

Takatori A, et al. (2008) Differential transmission of MEKK1 morphogenetic signals by JNK1 and JNK2. Development 135, 23-32
18032450   Curated Info

13

Das S, et al. (2005) Tpl2/cot signals activate ERK, JNK, and NF-kappaB in a cell-type and stimulus-specific manner. J Biol Chem 280, 23748-57
15833743   Curated Info

14

Leahy M, Lyons A, Krause D, O'Connor R (2004) Impaired Shc, Ras, and MAPK activation but normal Akt activation in FL5.12 cells expressing an insulin-like growth factor I receptor mutated at tyrosines 1250 and 1251. J Biol Chem 279, 18306-13
14963047   Curated Info

15

Jayanthi S, McCoy MT, Ladenheim B, Cadet JL (2002) Methamphetamine causes coordinate regulation of Src, Cas, Crk, and the Jun N-terminal kinase-Jun pathway. Mol Pharmacol 61, 1124-31
11961130   Curated Info

16

Vacratsis PO, Gallo KA (2000) Zipper-mediated oligomerization of the mixed lineage kinase SPRK/MLK-3 is not required for its activation by the GTPase cdc 42 but Is necessary for its activation of the JNK pathway. Monomeric SPRK L410P does not catalyze the activating phosphorylation of Thr258 of murine MITOGEN-ACTIVATED protein kinase kinase 4. J Biol Chem 275, 27893-900
10862766   Curated Info