Ser36
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Home > Phosphorylation Site Page: > Ser36  -  SHC1 (mouse)

Site Information
TPPEELPsPSASSLG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448508

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 10 ) , immunoprecipitation ( 2 , 5 ) , mutation of modification site ( 5 , 6 , 7 , 8 , 9 , 10 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 7 , 9 ) , western blotting ( 4 , 5 , 6 , 9 )
Disease tissue studied:
breast cancer ( 2 ) , kidney cancer ( 4 )
Relevant cell line - cell type - tissue:
'kidney, tubule' ( 3 ) , 'muscle, skeletal' ( 4 ) , 3T3 (fibroblast) ( 9 ) , BT-474 (breast cell) ( 2 ) , CHO (fibroblast) [JNK1 (human)] ( 10 ) , CHO (fibroblast) [MLK3 (human)] ( 10 ) , CHO (fibroblast) [SHC1 (mouse)] ( 10 ) , diaphragm ( 4 ) , fibroblast ( 4 ) , heart ( 4 , 5 ) , HeLa (cervical) ( 8 ) , kidney ( 4 , 6 ) , liver ( 4 ) , lung ( 4 ) , MDA-MB-231 (breast cell) ( 2 ) , MEF (fibroblast) ( 9 ) , MMC (mesangial) ( 7 ) , myocyte-heart ( 5 ) , NHMC (mesangial) ( 7 ) , Saos-2 (bone cell) ( 8 ) , skin ( 4 ) , TKPTS (renal tubule cell) ( 6 )

Upstream Regulation
Regulatory protein:
JNK1 (mouse) ( 6 ) , MEK1 (human) ( 3 )
Putative in vivo kinases:
ERK1 (mouse) ( 9 ) , ERK2 (mouse) ( 9 ) , JNK1 (human) ( 10 )
Kinases, in vitro:
ERK1 (human) ( 9 ) , JNK1 (human) ( 10 ) , JNK2 (human) ( 10 ) , JNK3 (human) ( 10 ) , P38A (human) ( 10 )
Treatments:
cisplatin ( 3 ) , development ( 4 ) , glucose ( 7 ) , H2O2 ( 2 , 6 , 9 ) , hyperglycemia ( 5 ) , ischemia/reperfusion ( 6 ) , JNK_inhibitor_I ( 9 ) , leptin ( 5 ) , oxidative_stress ( 4 ) , SB203580 ( 9 ) , serum ( 9 ) , U0126 ( 3 , 6 , 9 ) , UV ( 10 )

Downstream Regulation
Effects of modification on SHC1:
intracellular localization ( 5 ) , phosphorylation ( 6 , 9 )
Effects of modification on biological processes:
apoptosis, altered ( 5 , 8 , 9 ) , apoptosis, induced ( 3 , 6 , 7 ) , transcription, altered ( 9 ) , transcription, inhibited ( 6 )

References 

1

Kumar S, et al. (2017) Sirtuin1-regulated lysine acetylation of p66Shc governs diabetes-induced vascular oxidative stress and endothelial dysfunction. Proc Natl Acad Sci U S A 114, 1714-1719
28137876   Curated Info

2

Hudson J, et al. (2014) p66ShcA Promotes Breast Cancer Plasticity by Inducing an Epithelial-to-Mesenchymal Transition. Mol Cell Biol 34, 3689-701
25071152   Curated Info

3

Clark JS, et al. (2010) Cisplatin induces apoptosis through the ERK-p66shc pathway in renal proximal tubule cells. Cancer Lett 297, 165-70
20547441   Curated Info

4

Lebiedzinska M, et al. (2009) Age-related changes in levels of p66Shc and serine 36-phosphorylated p66Shc in organs and mouse tissues. Arch Biochem Biophys 486, 73-80
19327338   Curated Info

5

Malhotra A, et al. (2009) Inhibition of p66ShcA redox activity in cardiac muscle cells attenuates hyperglycemia-induced oxidative stress and apoptosis. Am J Physiol Heart Circ Physiol 296, H380-8
19060130   Curated Info

6

Arany I, Faisal A, Nagamine Y, Safirstein RL (2008) p66shc inhibits pro-survival epidermal growth factor receptor/ERK signaling during severe oxidative stress in mouse renal proximal tubule cells. J Biol Chem 283, 6110-7
18174162   Curated Info

7

Chintapalli J, et al. (2007) Inhibition of wild-type p66ShcA in mesangial cells prevents glycooxidant-dependent FOXO3a regulation and promotes the survival phenotype. Am J Physiol Renal Physiol 292, F523-30
17077388   Curated Info

8

Tiberi L, et al. (2006) p66(Shc) gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway. Biochem Biophys Res Commun 342, 503-8
16487929   Curated Info

9

Hu Y, et al. (2005) ERK phosphorylates p66shcA on Ser36 and subsequently regulates p27kip1 expression via the Akt-FOXO3a pathway: implication of p27kip1 in cell response to oxidative stress. Mol Biol Cell 16, 3705-18
15930121   Curated Info

10

Le S, Connors TJ, Maroney AC (2001) c-Jun N-terminal kinase specifically phosphorylates p66ShcA at serine 36 in response to ultraviolet irradiation. J Biol Chem 276, 48332-6
11602589   Curated Info