Ser465

Site Information
HNPIssVs_______ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448363

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 4 , 6 , 7 ) , phospho-antibody ( 1 , 3 , 8 ) , western blotting ( 1 , 3 , 8 )
Disease tissue studied:	brain cancer ( 8 ) , glioblastoma ( 8 ) , glioma ( 8 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 7 ) , C2C12 (myoblast) ( 3 ) , glial ( 8 ) , HEK293T (epithelial) ( 1 ) , HL-1 (myocyte) ( 4 ) , kidney ( 7 ) , liver ( 2 , 6 , 7 ) , lung ( 7 ) , spleen ( 7 ) , stem ( 8 ) , testis ( 7 ) , U-251 MG (glial) ( 8 )

Upstream Regulation
Regulatory protein:	ALK2 (human) ( 3 )
Putative upstream phosphatases:	PPM1H (mouse) ( 1 )
Treatments:	BMP2 ( 8 ) , CNTF ( 8 ) , LIF ( 8 )

References
1	Osawa J, et al. (2020) Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo. Biochem Biophys Res Commun 530, 513-519 32600616 Curated Info
2	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
3	Fujimoto M, et al. (2015) Mutant Activin-Like Kinase 2 in Fibrodysplasia Ossificans Progressiva are Activated via T203 by BMP Type II Receptors. Mol Endocrinol 29, 140-52 25354296 Curated Info
4	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
5	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
6	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
7	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
8	Lee J, et al. (2008) Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell 13, 69-80 18167341 Curated Info