Ser463
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Home > Phosphorylation Site Page: > Ser463  -  SMAD1 (mouse)

Site Information
SPHNPIssVs_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448362

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 4 , 5 , 6 ) , phospho-antibody ( 3 , 7 ) , western blotting ( 3 , 7 )
Disease tissue studied:
brain cancer ( 7 ) , glioblastoma ( 7 ) , glioma ( 7 )
Relevant cell line - cell type - tissue:
'fat, brown' ( 6 ) , C2C12 (myoblast) ( 3 ) , glial ( 7 ) , HL-1 (myocyte) ( 4 ) , kidney ( 6 ) , liver ( 2 , 5 , 6 ) , lung ( 6 ) , spleen ( 6 ) , stem ( 7 ) , testis ( 6 ) , U-251 MG (glial) ( 7 )

Upstream Regulation
Regulatory protein:
ALK2 (human) ( 3 )
Treatments:
BMP2 ( 7 ) , CNTF ( 7 ) , LIF ( 7 )

References 

1

Osawa J, et al. (2020) Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo. Biochem Biophys Res Commun 530, 513-519
32600616   Curated Info

2

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

3

Fujimoto M, et al. (2015) Mutant Activin-Like Kinase 2 in Fibrodysplasia Ossificans Progressiva are Activated via T203 by BMP Type II Receptors. Mol Endocrinol 29, 140-52
25354296   Curated Info

4

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

5

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

6

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

7

Lee J, et al. (2008) Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell 13, 69-80
18167341   Curated Info