Ser245

Site Information
VPLsLsPskRACAsP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4172657

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 )
Disease tissue studied:	breast cancer ( 5 ) , cervical cancer ( 8 ) , cervical adenocarcinoma ( 8 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 3 ) , BT-20 (breast cell) ( 5 ) , BT-549 (breast cell) ( 5 ) , Calu 6 (pulmonary) ( 5 ) , H2009 (pulmonary) ( 5 ) , H2077 (pulmonary) ( 5 ) , H2887 (pulmonary) ( 5 ) , H322M (pulmonary) ( 5 ) , HCC1359 (pulmonary) ( 5 ) , HCC1937 (breast cell) ( 5 ) , HCC2279 (pulmonary) ( 5 ) , HCC366 (pulmonary) ( 5 ) , HCC4006 (pulmonary) ( 5 ) , HCC78 (pulmonary) ( 5 ) , HeLa (cervical) ( 6 , 9 , 10 , 11 ) , HeLa S3 (cervical) ( 8 ) , HOP62 (pulmonary) ( 5 ) , HUES-9 ('stem, embryonic') ( 7 ) , MCF-7 (breast cell) ( 5 ) , MDA-MB-231 (breast cell) ( 5 ) , MDA-MB-468 (breast cell) ( 5 ) , NCI-H1395 (pulmonary) ( 5 ) , NCI-H1568 (pulmonary) ( 5 ) , NCI-H157 (pulmonary) ( 5 ) , NCI-H1648 (pulmonary) ( 5 ) , NCI-H1666 (pulmonary) ( 5 ) , NCI-H2030 (pulmonary) ( 5 ) , NCI-H2172 (pulmonary) ( 5 ) , NCI-H322 (pulmonary) ( 5 ) , NCI-H520 (squamous) ( 5 ) , NCI-H647 (pulmonary) ( 5 ) , PC9 (pulmonary) ( 5 ) , SH-SY5Y (neural crest) ( 2 ) , WM239A (melanocyte) ( 1 )

Upstream Regulation
Treatments:	EGF ( 11 ) , nocodazole ( 8 )

References
1	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
2	Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76 24117733 Curated Info
3	Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9 23836654 Curated Info
4	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
5	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
6	Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540 21857030 Curated Info
7	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
8	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
9	Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8 19276368 Curated Info
10	Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50 18452278 Curated Info
11	Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51 18220336 Curated Info