Tyr871
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Home > Phosphorylation Site Page: > Tyr871  -  EGFR (mouse)

Site Information
LGAEEkEyHAEGGkV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448061
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 5 , 7 , 11 , 12 , 13 ) , mutation of modification site ( 8 ) , phospho-antibody ( 2 , 3 , 4 , 8 , 9 , 10 , 14 ) , western blotting ( 2 , 3 , 4 , 8 , 9 , 10 )
Disease tissue studied:
bladder cancer ( 4 ) , liver cancer ( 3 ) , hepatocellular carcinoma ( 3 ) , lung cancer ( 2 , 7 ) , non-small cell lung cancer ( 2 , 7 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 11 , 12 , 13 ) , bladder ( 4 ) , epithelial ( 14 ) , HCC827 (pulmonary) ( 2 ) , hepatocyte-liver ( 3 ) , Huh7 (hepatic) ( 3 ) , lung ( 7 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) ( 2 , 5 , 8 , 9 ) , NCI-H1299 (pulmonary) ( 2 ) , NCI-H1650 (pulmonary) ( 2 ) , R22 ('muscle, smooth') ( 8 ) , SYF (fibroblast) ( 8 ) , vascular smooth muscle cell ('muscle, smooth') ( 8 )

Upstream Regulation
Putative in vivo kinases:
Src (rat) ( 8 ) , Src iso1 (mouse) ( 9 )
Putative upstream phosphatases:
DUSP3 (mouse) ( 2 )
Treatments:
cetuximab ( 4 ) , EGF ( 3 , 9 , 10 , 14 ) , estradiol ( 14 ) , faslodex ( 14 ) , light ( 4 ) , phenobarbital ( 3 ) , SB202190 ( 10 ) , TGF-beta ( 8 )

Downstream Regulation
Effects of modification on biological processes:
signaling pathway regulation ( 3 ) , transcription, induced ( 8 )

References 

1

Jin C, et al. (2022) MTDH-stabilized DDX17 promotes tumor initiation and progression through interacting with YB1 to induce EGFR transcription in Hepatocellular Carcinoma. Oncogene 42
36385375   Curated Info

2

Chen YR, et al. (2017) Deficiency in VHR/DUSP3, a suppressor of focal adhesion kinase, reveals its role in regulating cell adhesion and migration. Oncogene 36, 6509-6517
28759036   Curated Info

3

Mutoh S, et al. (2013) Phenobarbital indirectly activates the constitutive active androstane receptor (CAR) by inhibition of epidermal growth factor receptor signaling. Sci Signal 6, ra31
23652203   Curated Info

4

Bhuvaneswari R, Gan YY, Soo KC, Olivo M (2009) Targeting EGFR with photodynamic therapy in combination with Erbitux enhances in vivo bladder tumor response. Mol Cancer 8, 94
19878607   Curated Info

5

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

6

Rikova K (2008) CST Curation Set: 4437; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Rikova K (2008) CST Curation Set: 4438; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Samarakoon R, Higgins SP, Higgins CE, Higgins PJ (2008) TGF-beta1-induced plasminogen activator inhibitor-1 expression in vascular smooth muscle cells requires pp60(c-src)/EGFR(Y845) and Rho/ROCK signaling. J Mol Cell Cardiol 44, 527-38
18255094   Curated Info

9

Jo M, et al. (2007) Urokinase receptor primes cells to proliferate in response to epidermal growth factor. Oncogene 26, 2585-94
17043637   Curated Info

10

Frey MR, Dise RS, Edelblum KL, Polk DB (2006) p38 kinase regulates epidermal growth factor receptor downregulation and cellular migration. EMBO J 25, 5683-92
17139251   Curated Info

11

Guo A (2005) CST Curation Set: 630; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Guo A (2005) CST Curation Set: 631; Year: 2005; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Guo A (2005) CST Curation Set: 632; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Miyagawa S, Katsu Y, Watanabe H, Iguchi T (2004) Estrogen-independent activation of erbBs signaling and estrogen receptor alpha in the mouse vagina exposed neonatally to diethylstilbestrol. Oncogene 23, 340-9
14647453   Curated Info

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