Ser727

Site Information
tDNLLPMsPEEFDEM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447753

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 53 ) , flow cytometry ( 1 , 4 , 9 , 10 , 20 , 31 ) , immunoprecipitation ( 1 , 18 , 21 ) , mass spectrometry ( 19 , 21 , 22 , 24 , 27 ) , mutation of modification site ( 1 , 11 , 14 , 17 , 18 , 23 , 29 , 30 , 31 , 33 , 34 , 44 , 45 , 55 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 , 12 , 13 , 14 , 15 , 18 , 20 , 21 , 23 , 25 , 26 , 28 , 29 , 30 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 12 , 13 , 14 , 15 , 18 , 21 , 23 , 25 , 26 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 37 , 38 , 40 , 41 , 42 , 43 , 45 , 47 , 48 , 50 , 51 , 52 , 55 )
Disease tissue studied:	leukemia ( 43 ) , chronic myelogenous leukemia ( 43 ) , T cell leukemia ( 43 ) , liver cancer ( 23 , 29 ) , lymphoma ( 30 , 41 ) , anaplastic large cell lymphoma ( 41 ) , Burkitt's lymphoma ( 30 ) , neuroblastoma ( 3 ) , fibrosarcoma of soft tissue ( 8 , 30 , 34 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 293 (epithelial) ( 18 , 29 ) , 2fTGH (fibroblast) ( 8 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 27 ) , 32Dcl3 (myeloid) ( 27 ) , 3T3 (fibroblast) [STAT1 (mouse), homozygous knockout] ( 48 ) , 3T3 (fibroblast) ( 55 ) , B6 ( 9 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 19 ) , BMMC (mast) ( 25 ) , BV2 (microglia) ( 51 ) , C141 (epidermal) ( 41 ) , CD3+ (T lymphocyte) ( 11 ) , CD4+ (T lymphocyte) ( 2 , 14 ) , cortical progenitor ( 36 ) , endothelial-aorta ( 40 ) , fibroblast ( 6 , 49 ) , HEK293T (epithelial) ( 8 ) , HeLa (cervical) ( 8 , 18 ) , hematopoietic stem cells ( 20 ) , HepG2 (hepatic) ( 23 , 29 ) , J774 (macrophage) ( 33 , 47 ) , JB (epithelial) ( 38 ) , JB-6 (lymphocyte) ( 41 ) , JB6 CI41 (epidermal) ( 45 ) , KT-1 (myeloid) ( 43 ) , L929 (fibroblast) ( 39 ) , liver ( 7 ) , macrophage ( 13 ) , macrophage-bone marrow ( 1 , 11 , 15 , 21 , 23 , 44 ) , macrophage-peritoneum ( 1 , 24 ) , MEF (fibroblast) [Mnk1 (mouse), homozygous knockout] ( 26 ) , MEF (fibroblast) [Mnk2 (mouse), homozygous knockout] ( 26 ) , MEF (fibroblast) [P38A (mouse), homozygous knockout] ( 43 ) , MEF (fibroblast) [STAT1 (mouse), homozygous knockout] ( 31 ) , MEF (fibroblast) ( 5 , 8 , 23 , 28 , 29 , 30 , 41 , 42 , 52 ) , microglia ( 35 ) , MOLT-4 (T lymphocyte) ( 43 ) , mononuclear-blood ( 10 ) , myocyte-heart ( 32 ) , Neuro-2a (neuron) ( 3 ) , neuron ( 3 ) , neuron-'brain, cerebral cortex' ( 50 ) , neuron-'brain, cerebral cortex' [STAT1 (mouse), homozygous knockout] ( 50 ) , oocyte [CPEB (mouse)] ( 39 ) , osteoclast ( 37 ) , RAMOS (B lymphocyte) ( 30 ) , RAW 264 (macrophage) ( 46 , 53 ) , RAW 264.7 (macrophage) ( 1 , 12 , 34 ) , SCC16-5 (fibroblast) ( 54 ) , stromal ( 22 ) , T lymphocyte ( 4 ) , U3A (fibroblast) ( 30 , 34 )

Upstream Regulation
Regulatory protein:	BTRC (mouse) ( 30 ) , CDK7 (mouse) ( 23 ) , CDK8 (mouse) ( 23 ) , CDK9 (mouse) ( 23 ) , cGAS (mouse) ( 3 ) , eNOS (mouse) ( 32 ) , HEXB (mouse) ( 3 ) , KLRG1 (human) ( 9 ) , LATS1 (human) ( 8 ) , LATS2 (human) ( 8 ) , LY6C1 (mouse) ( 9 ) , METTL3 (human) ( 4 ) , MFAP5 (human) ( 6 ) , Mnk1 (mouse) ( 26 ) , Mnk2 (mouse) ( 26 ) , MTX2 (human) ( 7 ) , NPC1 (mouse) ( 3 ) , P38A (mouse) ( 45 ) , PKCD (mouse) ( 34 ) , SUMO1 (mouse) ( 25 ) , TFAM (human) ( 5 ) , TOP3A (human) ( 5 ) , ZBP1 (human) ( 5 )
Putative in vivo kinases:	CDK8 (human) ( 8 , 23 ) , ERK1 (human) ( 30 , 41 ) , ERK2 (mouse) ( 30 ) , JNK1 (mouse) ( 41 ) , P38A (mouse) ( 12 , 37 , 41 , 52 ) , P38B (human) ( 41 ) , RIPK1 (human) ( 5 ) , RIPK3 (human) ( 5 )
Kinases, in vitro:	CDK8 (mouse) ( 23 ) , ERK1 (human) ( 41 ) , ERK2 (mouse) ( 30 ) , JNK1 (human) ( 41 ) , P38A (human) ( 41 ) , P38B (human) ( 41 )
Putative upstream phosphatases:	SHP-2 (mouse) ( 49 )
Treatments:	actinomycin_D ( 23 ) , AG490 ( 33 , 47 ) , anisomycin ( 23 , 29 , 52 ) , antibody ( 10 ) , apigenin ( 33 , 47 ) , Apoptotic cells ( 10 ) , B-DNA ( 5 ) , BAPTA-AM ( 12 ) , cigarette_smoke ( 12 ) , DCA ( 14 ) , DETA-NONOate ( 32 ) , EGCG ( 38 ) , EGF ( 45 , 55 ) , flavopiridol ( 23 ) , ganglioside ( 51 ) , Go_6983 ( 8 ) , GSK'872 ( 5 ) , guanosine ( 35 ) , H2O2 ( 30 ) , IFN-alpha ( 8 , 28 , 43 ) , IFN-beta ( 8 , 23 , 29 , 48 , 53 ) , IFN-gamma ( 13 , 14 , 20 , 23 , 25 , 26 , 29 , 33 , 34 , 35 , 39 , 40 , 44 , 46 , 47 , 48 , 49 , 52 , 54 , 55 ) , IL-18 ( 9 ) , ischemia ( 22 ) , ischemia/reperfusion ( 32 , 50 ) , lactacystin ( 30 ) , LIF ( 36 ) , linoleic_acid ( 2 ) , LPS ( 1 , 21 , 23 , 40 , 46 , 48 , 53 ) , LY294002 ( 33 , 38 , 46 ) , MSC2530818 ( 8 ) , Necrostatin-1 ( 5 ) , olomoucine ( 23 ) , osmotic_stress ( 43 ) , PD169316 ( 41 ) , PD98059 ( 38 , 41 , 47 , 55 ) , RANKL ( 37 ) , rottlerin ( 34 , 46 ) , sanglifehrin A ( 13 ) , SB202190 ( 8 , 38 , 40 , 41 ) , SB203580 ( 12 , 30 , 37 , 46 , 52 , 55 ) , seliciclib ( 23 ) , serum ( 55 ) , siRNA ( 5 , 8 , 23 ) , SP600125 ( 38 ) , staurosporine ( 25 ) , Theaflavins ( 38 ) , TLR3/dsRNA complex inhibitor ( 4 ) , TNF ( 21 ) , U0126 ( 30 , 38 , 41 ) , UV ( 38 , 41 , 52 , 55 ) , vitronectin ( 20 ) , wortmannin ( 38 ) , Z-DNA ( 5 ) , Z-VAD-FMK ( 21 )

Downstream Regulation
Effects of modification on STAT1:	activity, induced ( 48 , 55 ) , activity, inhibited ( 25 ) , molecular association, regulation ( 13 , 30 ) , phosphorylation ( 23 ) , protein degradation ( 30 ) , sumoylation ( 25 )
Effects of modification on biological processes:	apoptosis, induced ( 12 ) , cell cycle regulation ( 31 ) , cell differentiation, induced ( 2 , 14 , 17 ) , cell growth, altered ( 45 ) , cell growth, inhibited ( 8 ) , cell motility, induced ( 11 ) , signaling pathway regulation ( 23 ) , transcription, altered ( 11 , 23 , 55 ) , transcription, induced ( 5 , 8 , 13 , 33 , 34 , 44 , 48 ) , transcription, inhibited ( 25 )
Induce interaction with:	BTRC (mouse) ( 30 ) , DNA ( 13 )

Disease / Diagnostics Relevance
Relevant diseases:	hypertrophic cardiomyopathy ( 11 ) , SLE ( 17 )

References
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