Tyr641
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Tyr641  -  STAT6 (mouse)

Site Information
MGKDGRGyVSTTIKM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447757

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 19 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 3 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 ) , modification-specific antibody ( 15 ) , mutation of modification site ( 2 , 15 , 19 ) , phospho-antibody ( 2 , 4 , 8 , 15 , 16 , 17 , 18 ) , phosphoamino acid analysis ( 19 ) , phosphopeptide mapping ( 19 ) , western blotting ( 2 , 4 , 8 , 18 )
Disease tissue studied:
leukemia ( 6 , 7 ) , chronic myelogenous leukemia ( 6 , 7 ) , lymphoma ( 19 ) , B cell lymphoma ( 19 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 15 , 19 ) , 3T3 (fibroblast) ( 8 ) , B lymphocyte [ECM1 (mouse), homozygous knockout] ( 4 ) , B lymphocyte-spleen ( 19 ) , BaF3 ('B lymphocyte, precursor') ( 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 ) , fibroblast-lung ( 8 ) , macrophage-bone marrow ( 2 ) , mast-bone marrow ( 17 ) , MEF (fibroblast) ( 18 ) , RAW 264.7 (macrophage) ( 3 ) , T lymphocyte [ECM1 (mouse), homozygous knockout, KO] ( 4 ) , T lymphocyte-blood ( 16 )

Upstream Regulation
Regulatory protein:
STAT6 (mouse) ( 4 )
Treatments:
cardiotropin-1 ( 8 ) , H-7 ( 19 ) , HA-1004 ( 19 ) , IL-11 ( 8 ) , IL-4 ( 8 , 16 , 17 , 18 , 19 ) , IL-6 ( 8 ) , LIF ( 8 ) , LY294002 ( 19 ) , oncostatin_M ( 8 ) , staurosporine ( 18 ) , wortmannin ( 19 )

Downstream Regulation
Effects of modification on STAT6:
intracellular localization ( 17 ) , protein degradation ( 17 )

References 

1

McKay ZP, Brown MC, Gromeier M (2021) Aryl Hydrocarbon Receptor Signaling Controls CD155 Expression on Macrophages and Mediates Tumor Immunosuppression. J Immunol
33504618   Curated Info

2

Li X, et al. (2017) Myeloid-derived cullin 3 promotes STAT3 phosphorylation by inhibiting OGT expression and protects against intestinal inflammation. J Exp Med 214, 1093-1109
28280036   Curated Info

3

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

4

Li Z, et al. (2011) ECM1 controls T(H)2 cell egress from lymph nodes through re-expression of S1P(1). Nat Immunol 12, 178-85
21217760   Curated Info

5

Rikova K (2008) CST Curation Set: 4478; Year: 2008; Biosample/Treatment: cell line, MD Ander 0895/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Gu T (2007) CST Curation Set: 3402; Year: 2007; Biosample/Treatment: cell line, Baf3(MSCV-NEO)/IL3 withdrawal; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Gu T (2007) CST Curation Set: 3406; Year: 2007; Biosample/Treatment: cell line, Baf3(PLXSN-NEO-FLT3/ITD)/IL3 withdrawal; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Fritz DK, et al. (2006) Oncostatin-M up-regulates VCAM-1 and synergizes with IL-4 in eotaxin expression: involvement of STAT6. J Immunol 176, 4352-60
16547273   Curated Info

9

Gu T (2005) CST Curation Set: 1059; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Goss V (2005) CST Curation Set: 847; Year: 2005; Biosample/Treatment: cell line, BaF3/serum starved; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Goss V (2005) CST Curation Set: 848; Year: 2005; Biosample/Treatment: cell line, BaF3/serum starved; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Gu T (2005) CST Curation Set: 845; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Gu T (2005) CST Curation Set: 700; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Goss V (2004) CST Curation Set: 494; Year: 2004; Biosample/Treatment: cell line, BaF3/serum starved &'||' Tpo; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Chen W, Daines MO, Hershey GK (2004) Methylation of STAT6 modulates STAT6 phosphorylation, nuclear translocation, and DNA-binding activity. J Immunol 172, 6744-50
15153491   Curated Info

16

Blaeser F, et al. (2003) Targeted inactivation of the IL-4 receptor alpha chain I4R motif promotes allergic airway inflammation. J Exp Med 198, 1189-200
14557412   Curated Info

17

Sherman MA, Powell DR, Brown MA (2002) IL-4 induces the proteolytic processing of mast cell STAT6. J Immunol 169, 3811-8
12244176   Curated Info

18

ten Hoeve J, et al. (2002) Identification of a nuclear Stat1 protein tyrosine phosphatase. Mol Cell Biol 22, 5662-8
12138178   Curated Info

19

Wick KR, Berton MT (2000) IL-4 induces serine phosphorylation of the STAT6 transactivation domain in B lymphocytes. Mol Immunol 37, 641-52
11164892   Curated Info