Tyr1055
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Home > Phosphorylation Site Page: > Tyr1055  -  iNOS (human)

Site Information
LPGKPKVyVQDILRQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 4115776

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 , 5 , 6 ) , mutation of modification site ( 4 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Disease tissue studied:
colorectal cancer, surrounding tissue ( 2 ) , lung cancer ( 3 , 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , A549 (pulmonary) ( 4 ) , colon ( 2 ) , HeLa (cervical) ( 4 ) , lung ( 3 ) , NHBE (epithelial) ( 4 )

Upstream Regulation
Regulatory protein:
EGFR (human) ( 4 )
Putative in vivo kinases:
Src (human) ( 4 )
Treatments:
cytokines ( 4 ) , EGF ( 4 ) , vanadate ( 4 )

Downstream Regulation
Effects of modification on iNOS:
protein stabilization ( 4 )

References 

1

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

2

Tucker M (2010) CST Curation Set: 10853; Year: 2010; Biosample/Treatment: tissue, colon/untreated; Disease: colorectal cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Hu Y (2010) CST Curation Set: 8945; Year: 2010; Biosample/Treatment: tissue, lung/untreated; Disease: lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Tyryshkin A, et al. (2010) Src kinase-mediated phosphorylation stabilizes inducible nitric-oxide synthase in normal cells and cancer cells. J Biol Chem 285, 784-92
19875457   Curated Info

5

Li Y (2008) CST Curation Set: 4372; Year: 2008; Biosample/Treatment: tissue, colon/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Gu T (2007) CST Curation Set: 3070; Year: 2007; Biosample/Treatment: tissue, bone marrow/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info