Ser363
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Home > Phosphorylation Site Page: > Ser363  -  DOR-1 (human)

Site Information
RVtACtPsDGPGGGA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448550

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 4 , 6 , 7 , 8 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 3 , 4 , 5 , 6 , 8 )
Disease tissue studied:
breast cancer ( 4 ) , neuroblastoma ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
GRK5 (human) ( 1 ) , GRK6 (human) ( 1 )
Putative in vivo kinases:
GRK2 (human) ( 1 , 5 ) , GRK3 (human) ( 1 )
Putative upstream phosphatases:
PPP1CA (human) ( 1 ) , PPP1CB (human) ( 1 )
Treatments:
AG1478 ( 4 ) , bisindolylmaleimide ( 5 ) , BW373U86 ( 3 ) , calyculin_A ( 1 ) , Cmpd101 ( 1 ) , DADLE ( 1 ) , deltorphin_I ( 1 , 5 ) , deltorphin_II ( 1 , 3 , 6 , 7 , 8 ) , DPDPE ( 1 , 3 , 4 , 5 ) , EGF ( 4 ) , etorphine ( 5 ) , fentanyl ( 1 ) , morphine ( 1 , 3 , 7 ) , naloxone ( 1 ) , naltrexone ( 1 ) , naltrindole ( 1 , 5 , 7 ) , NKH_477 ( 1 ) , okadaic_acid ( 1 ) , OMI ( 3 ) , phorbol_ester ( 1 ) , PTX ( 3 ) , siRNA ( 1 ) , TAN-67 ( 3 )

Downstream Regulation
Effects of modification on DOR-1:
receptor desensitization, altered ( 6 , 8 ) , receptor inactivation, induced ( 1 ) , receptor internalization, altered ( 8 ) , receptor internalization, induced ( 1 )

References 

1

Mann A, et al. (2020) Agonist-induced phosphorylation bar code and differential post-activation signaling of the delta opioid receptor revealed by phosphosite-specific antibodies. Sci Rep 10, 8585
32444688   Curated Info

2

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

3

Bradbury FA, Zelnik JC, Traynor JR (2009) G protein independent phosphorylation and internalization of the delta-opioid receptor. J Neurochem 109, 1526-35
19344370   Curated Info

4

Chen Y, et al. (2008) EGF Transregulates Opioid Receptors through EGFR-mediated GRK2 Phosphorylation and Activation. Mol Biol Cell 19, 2973-83
18463167   Curated Info

5

Marie N, et al. (2008) Different kinases desensitize the human delta-opioid receptor (hDOP-R) in the neuroblastoma cell line SK-N-BE upon peptidic and alkaloid agonists. Cell Signal 20, 1209-20
18395423   Curated Info

6

Navratilova E, et al. (2007) Quantitative evaluation of human delta opioid receptor desensitization using the operational model of drug action. Mol Pharmacol 71, 1416-26
17322005   Curated Info

7

Navratilova E, et al. (2005) Morphine promotes phosphorylation of the human delta-opioid receptor at serine 363. Eur J Pharmacol 519, 212-4
16135360   Curated Info

8

Law PY, et al. (2000) Deltorphin II-induced rapid desensitization of delta-opioid receptor requires both phosphorylation and internalization of the receptor. J Biol Chem 275, 32057-65
10893226   Curated Info