Lys382
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Home > Ubiquitylation Site Page: > Lys382  -  p53 (human)

Site Information
QstsRHkkLMFktEG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 464658

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 4 , 5 ) , microscopy-colocalization with upstream kinase ( 1 ) , mutation of modification site ( 2 , 4 , 5 , 7 ) , western blotting ( 1 , 3 , 4 , 5 , 7 )
Disease tissue studied:
bone cancer ( 3 , 5 , 7 ) , colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lung cancer ( 2 , 3 , 4 , 7 ) , non-small cell lung cancer ( 2 , 3 , 4 , 7 ) , neuroblastoma ( 3 ) , ovarian epithelial carcinoma ( 1 )
Relevant cell line - cell type - tissue:
HCT116 (intestinal) ( 3 ) , HEK293T (epithelial) ( 1 ) , LAN-5 (neural crest) ( 3 ) , NCI-H1299 (pulmonary) ( 2 , 3 , 4 , 7 ) , RKO (intestinal) ( 3 ) , Saos-2 (bone cell) ( 5 , 7 ) , TOV-21G (epithelial) ( 1 ) , U2OS (bone cell) ( 3 , 5 )

Upstream Regulation
Regulatory protein:
ING1 (human) ( 2 ) , MDM4 (human) ( 4 )
Treatments:
MG132 ( 7 )

Downstream Regulation
Effects of modification on p53:
intracellular localization ( 1 , 5 ) , molecular association, regulation ( 2 ) , protein degradation ( 1 , 2 , 3 , 5 , 7 )
Effects of modification on biological processes:
transcription, inhibited ( 7 )
Inhibit interaction with:
ING1 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
ovarian epithelial carcinoma ( 1 )

References 

1

Zhang L, et al. (2019) SMYD3 promotes epithelial ovarian cancer metastasis by downregulating p53 protein stability and promoting p53 ubiquitination. Carcinogenesis 40, 1492-1503
31002112   Curated Info

2

Thalappilly S, et al. (2011) The p53 tumor suppressor is stabilized by inhibitor of growth 1 (ING1) by blocking polyubiquitination. PLoS One 6, e21065
21731648   Curated Info

3

Marchenko ND, et al. (2010) Stress-mediated nuclear stabilization of p53 is regulated by ubiquitination and importin-alpha3 binding. Cell Death Differ 17, 255-67
19927155   Curated Info

4

Okamoto K, Taya Y, Nakagama H (2009) Mdmx enhances p53 ubiquitination by altering the substrate preference of the Mdm2 ubiquitin ligase. FEBS Lett 583, 2710-4
19619542   Curated Info

5

Nie L, Sasaki M, Maki CG (2007) Regulation of p53 nuclear export through sequential changes in conformation and ubiquitination. J Biol Chem 282, 14616-25
17371868   Curated Info

6

Ito A, et al. (2002) MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation. EMBO J 21, 6236-45
12426395   Curated Info

7

Rodriguez MS, et al. (2000) Multiple C-terminal lysine residues target p53 for ubiquitin-proteasome-mediated degradation. Mol Cell Biol 20, 8458-67
11046142   Curated Info

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