Thr393
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Home > Phosphorylation Site Page: > Thr393  -  CCNE1 (mouse)

Site Information
PPPsVVLtPPPSSKK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447676

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 9 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 2 , 5 , 7 ) , microscopy-colocalization with upstream kinase ( 8 ) , mutation of modification site ( 3 , 4 , 8 ) , phospho-antibody ( 6 , 8 , 9 ) , western blotting ( 3 , 6 , 8 , 9 )
Disease tissue studied:
liver cancer ( 4 ) , squamous cell carcinoma of the liver ( 4 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 5 ) , 'stem, hematopoietic' ( 4 ) , bone marrow ( 3 ) , embryo ( 6 ) , erythroblast-spleen ( 4 ) , HSC (squamous) ( 4 ) , liver ( 7 ) , MEF (fibroblast) ( 8 ) , MEF (fibroblast) [CCNE1 (mouse), genetic knockin] ( 9 ) , MEF (fibroblast) [p21Cip1 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , spleen ( 7 ) , thymocyte-thymus ( 3 )

Upstream Regulation
Regulatory protein:
FBXW7 iso3 (mouse) ( 6 ) , GLMN (mouse) ( 6 )
Treatments:
insulin ( 5 )

Downstream Regulation
Effects of modification on CCNE1:
activity, inhibited ( 8 ) , enzymatic activity, inhibited ( 9 ) , methylation ( 4 ) , phosphorylation ( 4 ) , protein degradation ( 3 , 4 , 6 , 8 , 9 ) , ubiquitination ( 3 , 4 )
Effects of modification on biological processes:
apoptosis, inhibited ( 4 ) , carcinogenesis, inhibited ( 9 ) , cell cycle regulation ( 3 , 4 , 8 , 9 ) , cell differentiation, altered ( 8 ) , cell differentiation, induced ( 4 ) , cell growth, altered ( 8 , 9 ) , DNA repair, altered ( 4 ) , transcription, altered ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 9 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

3

Siu KT, et al. (2014) Chromosome instability underlies hematopoietic stem cell dysfunction and lymphoid neoplasia associated with impaired fbw7-mediated cyclin e regulation. Mol Cell Biol 34, 3244-58
24958101   Curated Info

4

Xu Y, et al. (2014) Fbw7-dependent cyclin E regulation ensures terminal maturation of bone marrow erythroid cells by restraining oxidative metabolism. Oncogene 33, 3161-71
23873023   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Tron AE, et al. (2012) The glomuvenous malformation protein Glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Mol Cell 46, 67-78
22405651   Curated Info

7

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

8

Minella AC, et al. (2008) Cyclin E phosphorylation regulates cell proliferation in hematopoietic and epithelial lineages in vivo. Genes Dev 22, 1677-89
18559482   Curated Info

9

Loeb KR, et al. (2005) A mouse model for cyclin E-dependent genetic instability and tumorigenesis. Cancer Cell 8, 35-47
16023597   Curated Info