Ser261
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Home > Phosphorylation Site Page: > Ser261  -  AQP2 (mouse)

Site Information
RQsVELHsPQsLPRG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 464644

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 5 , 7 ) , mutation of modification site ( 3 , 4 , 8 , 10 , 11 ) , phospho-antibody ( 1 , 2 , 3 , 6 , 9 , 10 , 11 , 12 ) , western blotting ( 1 , 2 , 3 , 6 , 8 , 9 , 10 )
Disease tissue studied:
kidney cancer ( 9 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 1 ) , HEK293T (epithelial) ( 4 ) , kidney ( 5 , 9 , 11 , 12 ) , LLC-PK1 (renal) ( 3 ) , MDCK (epithelial) ( 8 , 11 ) , mpkCCD (renal) ( 6 , 7 ) , oocyte ( 10 )

Upstream Regulation
Putative in vivo kinases:
P38A (mouse) ( 1 )
Treatments:
desmopressin ( 2 , 11 ) , erlotinib ( 3 ) , NH4Cl ( 9 ) , NPS_2143 ( 1 ) , SB203580 ( 1 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 8 ) , ubiquitination ( 1 )

References 

1

Ranieri M, et al. (2017) CaSR signaling down-regulates AQP2 expression via a novel microRNA pathway in pendrin and NaCl cotransporter knockout mice. FASEB J
29212817   Curated Info

2

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

3

Cheung PW, et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol 27, 3105-3116
27694161   Curated Info

4

Moeller HB, et al. (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. J Biol Chem 291, 2469-84
26645691   Curated Info

5

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

6

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

7

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

8

Moeller HB, Praetorius J, Rützler MR, Fenton RA (2010) Phosphorylation of aquaporin-2 regulates its endocytosis and protein-protein interactions. Proc Natl Acad Sci U S A 107, 424-9
19966308   Curated Info

9

Nowik M, et al. (2010) Induction of metabolic acidosis with ammonium chloride (NH4Cl) in mice and rats--species differences and technical considerations. Cell Physiol Biochem 26, 1059-72
21220937   Curated Info

10

Moeller HB, Macaulay N, Knepper MA, Fenton RA (2009) Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating. Am J Physiol Renal Physiol 296, F649-57
19144687   Curated Info

11

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

12

Hoffert JD, et al. (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292, F691-700
16985212   Curated Info