Ser261
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Home > Phosphorylation Site Page: > Ser261  -  AQP2 (human)

Site Information
RQsVELHsPQsLPRG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 464644

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 5 ) , immunoassay ( 5 ) , immunoprecipitation ( 2 , 4 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 , 3 , 5 , 6 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 )
Disease tissue studied:
leukemia ( 2 )
Relevant cell line - cell type - tissue:
'kidney, cortex' ( 4 ) , 'kidney, inner medulla' ( 4 ) , 'kidney, outer medulla' ( 4 ) , E.coli (bacterial) ( 3 ) , M1 (myeloid) ( 2 ) , MCD4 (renal) ( 2 ) , MDCK (epithelial) ( 4 , 5 ) , mpkCCD (renal) ( 5 , 6 ) , Pichia pastoris (yeast cell) ( 3 ) , urine ( 1 )

Upstream Regulation
Regulatory protein:
CHIP (human) ( 2 ) , PKD1 (human) ( 4 )
Treatments:
ATP ( 6 ) , calyculin_A ( 4 ) , dDAVP ( 4 ) , desmopressin ( 6 ) , dopamine ( 6 ) , FR180204 ( 2 ) , NKH_477 ( 4 , 5 ) , seliciclib ( 4 ) , vasopressin ( 1 , 5 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 5 ) , molecular association, regulation ( 2 , 3 )
Effects of modification on biological processes:
exocytosis, induced ( 1 )
Inhibit interaction with:
HSP70 (human) ( 2 ) , VTA1 (mouse) ( 3 )

References 

1

Sakai M, et al. (2020) Phosphorylation profile of human AQP2 in urinary exosomes by LC-MS/MS phosphoproteomic analysis. Clin Exp Nephrol
32529500   Curated Info

2

Centrone M, et al. (2017) AQP2 Abundance is Regulated by the E3-Ligase CHIP Via HSP70. Cell Physiol Biochem 44, 515-531
29145196   Curated Info

3

Roche JV, et al. (2017) Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5. J Biol Chem 292, 14636-14648
28710278   Curated Info

4

Tamma G, et al. (2014) A protein kinase A-independent pathway controlling aquaporin 2 trafficking as a possible cause for the syndrome of inappropriate antidiuresis associated with polycystic kidney disease 1 haploinsufficiency. J Am Soc Nephrol 25, 2241-53
24700872   Curated Info

5

Trimpert C, et al. (2012) Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus. Nephrol Dial Transplant 27, 4389-97
22778181   Curated Info

6

Boone M, et al. (2011) Counteracting vasopressin-mediated water reabsorption by ATP, dopamine, and phorbol esters: mechanisms of action. Am J Physiol Renal Physiol 300, F761-71
21209006   Curated Info