Ser261
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Home > Phosphorylation Site Page: > Ser261  -  AQP2 (rat)

Site Information
RQsVELHsPQsLPRG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 464644

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 19 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 17 , 24 , 25 , 28 , 30 ) , mutation of modification site ( 18 , 19 , 27 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 16 , 19 , 20 , 22 , 23 , 25 , 26 , 29 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 13 , 16 , 19 , 20 , 22 , 23 , 26 , 27 )
Disease tissue studied:
kidney cancer ( 20 , 23 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 6 , 8 , 9 , 10 , 13 , 22 , 24 , 25 , 26 , 28 , 30 ) , IMCD ( 16 , 19 ) , kidney ( 12 , 17 , 20 , 23 , 29 ) , LLC-PK1 (renal) ( 2 , 4 , 7 , 18 , 27 ) , MDCK (epithelial) ( 3 , 5 , 12 ) , mpkCCD (renal) ( 22 )

Upstream Regulation
Regulatory protein:
ILK (rat) ( 7 )
Treatments:
4AD ( 16 ) , BUO ( 19 ) , calyculin_A ( 8 , 9 , 14 ) , ciclosporin ( 4 ) , colforsin ( 5 , 14 , 16 , 27 ) , cpd22 ( 7 ) , desmopressin ( 6 , 11 , 25 , 26 , 28 , 29 , 30 ) , FK506 ( 8 , 9 ) , IBMX ( 6 ) , meloxicam ( 13 ) , Mn(2+) ( 2 ) , NH4Cl ( 23 ) , NKH_477 ( 3 ) , okadaic_acid ( 4 ) , PD98059 ( 4 ) , roflumilast ( 6 ) , roflumilast N-oxide ( 6 ) , sanguinarine ( 4 ) , seliciclib ( 14 ) , sulprostone ( 10 ) , vasopressin ( 2 , 4 , 7 , 12 , 27 )

Downstream Regulation
Effects of modification on AQP2:
intracellular localization ( 3 , 12 , 19 , 21 ) , molecular association, regulation ( 24 ) , ubiquitination ( 8 )
Effects of modification on biological processes:
exocytosis, induced ( 8 )
Inhibit interaction with:
ANXA2 (rat) ( 24 ) , GDI2 (rat) ( 24 ) , GRP78 (rat) ( 24 ) , HSC70 (rat) ( 24 ) , HSP70 (rat) ( 24 ) , PPP1CA (rat) ( 24 ) , RAN (rat) ( 24 )

References 

1

Liwang J, et al. (2018) Role of PKC and AMPK in hypertonicity-stimulated water reabsorption in rat inner medullary collecting ducts. Am J Physiol Renal Physiol
30427219   Curated Info

2

Lei L, et al. (2017) Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. Am J Physiol Renal Physiol, ajprenal003912017
29046300   Curated Info

3

Yui N, Ando F, Sasaki S, Uchida S (2017) Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochem Biophys Res Commun 490, 1039-1044
28668390   Curated Info

4

Cheung PW, et al. (2017) Protein phosphatase 2C is responsible for VP-induced dephosphorylation of AQP2 serine 261. Am J Physiol Renal Physiol 313, F404-F413
28381458   Curated Info

5

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

6

Umejiego EN, Wang Y, Knepper MA, Chou CL (2017) Roflumilast and aquaporin-2 regulation in rat renal inner medullary collecting duct. Physiol Rep 5
28108651   Curated Info

7

Mamuya FA, et al. (2016) ILK and cytoskeletal architecture: an important determinant of AQP2 recycling and subsequent entry into the exocytotic pathway. Am J Physiol Renal Physiol 311, F1346-F1357
27760768   Curated Info

8

Ren H, et al. (2016) Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane. Am J Physiol Renal Physiol 311, F1189-F1197
27488997   Curated Info

9

Al-Bataineh MM, et al. (2016) Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells. Am J Physiol Renal Physiol 311, F890-F900
27534994   Curated Info

10

Hao S, et al. (2016) The EP3 receptor regulates water excretion in response to high salt intake. Am J Physiol Renal Physiol 311, F822-F829
27465993   Curated Info

11

Choi HJ, Jung HJ, Kwon TH (2015) Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308, F737-48
25651562   Curated Info

12

Rice WL, et al. (2015) Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PLoS One 10, e0131719
26147297   Curated Info

13

Ren H, et al. (2015) NSAIDs Alter Phosphorylated Forms of AQP2 in the Inner Medullary Tip. PLoS One 10, e0141714
26517129   Curated Info

14

Tamma G, et al. (2014) A protein kinase A-independent pathway controlling aquaporin 2 trafficking as a possible cause for the syndrome of inappropriate antidiuresis associated with polycystic kidney disease 1 haploinsufficiency. J Am Soc Nephrol 25, 2241-53
24700872   Curated Info

15

Miranda CA, Lee JW, Chou CL, Knepper MA (2014) Tolvaptan as a tool in renal physiology. Am J Physiol Renal Physiol 306, F359-66
24305472   Curated Info

16

Bogum J, et al. (2013) Small-molecule screening identifies modulators of aquaporin-2 trafficking. J Am Soc Nephrol 24, 744-58
23559583   Curated Info

17

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

18

Rice WL, et al. (2012) Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS One 7, e32843
22403603   Curated Info

19

Stødkilde L, et al. (2011) Bilateral ureteral obstruction induces early downregulation and redistribution of AQP2 and phosphorylated AQP2. Am J Physiol Renal Physiol 301, F226-35
21525134   Curated Info

20

Boone M, et al. (2011) Counteracting vasopressin-mediated water reabsorption by ATP, dopamine, and phorbol esters: mechanisms of action. Am J Physiol Renal Physiol 300, F761-71
21209006   Curated Info

21

Tamma G, et al. (2011) Regulation of AQP2 localization by S256 and S261 phosphorylation and ubiquitination. Am J Physiol Cell Physiol 300, C636-46
21148409   Curated Info

22

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

23

Nowik M, et al. (2010) Induction of metabolic acidosis with ammonium chloride (NH4Cl) in mice and rats--species differences and technical considerations. Cell Physiol Biochem 26, 1059-72
21220937   Curated Info

24

Zwang NA, et al. (2009) Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry. J Proteome Res 8, 1540-54
19209902   Curated Info

25

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

26

Yu MJ, et al. (2008) Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct. Am J Physiol Cell Physiol 295, C661-78
18596208   Curated Info

27

Lu HJ, et al. (2008) The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells. Am J Physiol Renal Physiol 295, F290-4
18434387   Curated Info

28

Hoffert JD, et al. (2007) An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins. J Proteome Res 6, 3501-8
17683130   Curated Info

29

Hoffert JD, et al. (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292, F691-700
16985212   Curated Info

30

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info