Ser368
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Home > Phosphorylation Site Page: > Ser368  -  GJA1 (mouse)

Site Information
QRPssRAssRAssRP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447813

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 , 9 ) , immunoprecipitation ( 9 , 10 ) , mass spectrometry ( 11 , 12 , 13 , 14 ) , microscopy-colocalization with upstream kinase ( 7 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 15 , 16 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 8 , 9 , 10 , 16 )
Disease tissue studied:
melanoma skin cancer ( 14 )
Relevant cell line - cell type - tissue:
'heart, ventricle' ( 8 , 10 ) , brain ( 5 , 11 , 12 , 13 ) , HaCaT (keratinocyte) ( 9 ) , heart ( 1 , 2 , 9 , 12 ) , Leydig TM3 (Leydig) ( 3 ) , myocyte-heart ( 2 , 4 , 8 ) , ovary ( 7 , 15 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 14 ) , testis ( 12 ) , TM4 (sertoli) ( 6 ) , TtT/GF (pituitary cell) ( 16 )

Upstream Regulation
Regulatory protein:
MFN1 (human) ( 1 ) , Notch 1 (human) ( 2 )
Treatments:
2-APB ( 8 ) , ATP ( 8 ) , benzo(a)pyrene ( 6 ) , FK506 ( 10 ) , IL-1b ( 16 ) , ischemia ( 9 ) , lactacystin ( 16 ) , leupeptin ( 16 ) , LH ( 15 ) , lindane ( 3 ) , methoxychlor ( 3 ) , phorbol_ester ( 3 , 9 ) , ponatinib ( 2 ) , TNF ( 16 ) , triclocarban ( 3 ) , triclosan ( 3 ) , XeC ( 8 )

Downstream Regulation
Effects of modification on GJA1:
protein degradation ( 9 ) , protein stabilization ( 10 ) , ubiquitination ( 9 )
Effects of modification on biological processes:
endocytosis, induced ( 9 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertrophic cardiomyopathy ( 10 )

References 

1

Kwek XY, et al. (2022) Role of cardiac mitofusins in cardiac conduction following simulated ischemia-reperfusion. Sci Rep 12, 21049
36473917   Curated Info

2

Madonna R, et al. (2021) Connexin 43 and Connexin 26 Involvement in the Ponatinib-Induced Cardiomyopathy: Sex-Related Differences in a Murine Model. Int J Mol Sci 22
34071707   Curated Info

3

Yawer A, et al. (2020) Endocrine-disrupting chemicals rapidly affect intercellular signaling in Leydig cells. Toxicol Appl Pharmacol 404, 115177
32739526   Curated Info

4

Sun Z, et al. (2019) Connexin 43-serine 282 modulates serine 279 phosphorylation in cardiomyocytes. Biochem Biophys Res Commun 513, 567-572
30981509   Curated Info

5

Freitas-Andrade M, et al. (2019) Targeting MAPK phosphorylation of Connexin43 provides neuroprotection in stroke. J Exp Med 216, 916-935
30872361   Curated Info

6

Ji X, et al. (2018) Benzo[a]pyrene-decreased gap junctional intercellular communication via calcium/calmodulin signaling increases apoptosis in TM4 cells. J Appl Toxicol
29578247   Curated Info

7

Norris RP, Baena V, Terasaki M (2017) Localization of phosphorylated connexin 43 using serial section immunogold electron microscopy. J Cell Sci 130, 1333-1340
28202692   Curated Info

8

Kang M, et al. (2014) Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP3/IP3 receptor signaling. Cell Commun Signal 12, 58
25262337   Curated Info

9

Smyth JW, et al. (2014) A 14-3-3 mode-1 binding motif initiates gap junction internalization during acute cardiac ischemia. Traffic 15, 684-99
24612377   Curated Info

10

Fontes MS, et al. (2014) Changes in Cx43 and NaV1.5 expression precede the occurrence of substantial fibrosis in calcineurin-induced murine cardiac hypertrophy. PLoS One 9, e87226
24498049   Curated Info

11

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

12

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

13

Zhou J (2010) CST Curation Set: 9271; Year: 2010; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)XpS
Curated Info

14

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

15

Norris RP, et al. (2008) Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption. Development 135, 3229-38
18776144   Curated Info

16

Fortin ME, Pelletier RM, Meilleur MA, Vitale ML (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF. Biol Reprod 74, 2-12
16135697   Curated Info

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