Thr389
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr389  -  p70S6K iso2 (human)

Site Information
NQVFLGFtYVAPSVL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448607

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 9 ) , mutation of modification site ( 9 , 12 ) , phospho-antibody ( 5 , 6 , 8 , 13 ) , western blotting ( 5 , 6 , 8 , 9 , 13 )
Disease tissue studied:
breast cancer ( 6 ) , pancreatic cancer ( 13 ) , pancreatic carcinoma ( 13 )
Relevant cell line - cell type - tissue:
fibroblast-embryo ( 9 ) , HEK293T (epithelial) ( 8 ) , HeLa (cervical) ( 6 ) , MDA-MB-435 (breast cell) ( 6 ) , pancreas ( 13 ) , SF9 ( 12 ) , VSMC ( 5 )

Upstream Regulation
Regulatory protein:
IKKB (human) ( 5 )
Kinases, in vitro:
mTOR (human) ( 6 )
Treatments:
angiotensin_2 ( 5 ) , EGF ( 8 ) , glucose_starvation ( 9 ) , L-glutamine_withdrawal ( 9 ) , LPS ( 10 ) , ML120B ( 5 ) , nicotinamide ( 4 ) , QLT0254 ( 13 ) , rapamycin ( 9 , 10 ) , SB216763 ( 10 ) , Torin1 ( 4 )

Downstream Regulation
Effects of modification on p70S6K iso2:
enzymatic activity, induced ( 12 )

References 

1

Miller WP, et al. (2020) The stress response protein REDD1 promotes diabetes-induced oxidative stress in the retina by Keap1-independent Nrf2 degradation. J Biol Chem
32295843   Curated Info

2

Arif A, et al. (2019) Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol Cell 73, 446-457.e6
30612880   Curated Info

3

Deng L, et al. (2015) The Ubiquitination of RagA GTPase by RNF152 Negatively Regulates mTORC1 Activation. Mol Cell 58, 804-18
25936802   Curated Info

4

Hong S, et al. (2014) Cross-talk between sirtuin and mammalian target of rapamycin complex 1 (mTORC1) signaling in the regulation of S6 kinase 1 (S6K1) phosphorylation. J Biol Chem 289, 13132-41
24652283   Curated Info

5

Doyon P, van Zuylen WJ, Servant MJ (2013) Role of IκB Kinase-β in the Growth-Promoting Effects of Angiotensin II In Vitro and In Vivo. Arterioscler Thromb Vasc Biol 33, 2850-7
24135021   Curated Info

6

Chen CH, et al. (2013) Autoregulation of the mechanistic target of rapamycin (mTOR) complex 2 integrity is controlled by an ATP-dependent mechanism. J Biol Chem 288, 27019-30
23928304   Curated Info

7

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

8

Tumurkhuu M, et al. (2013) A novel SOS1 mutation in Costello/CFC syndrome affects signaling in both RAS and PI3K pathways. J Recept Signal Transduct Res 33, 124-8
23528009   Curated Info

9

Kim SG, et al. (2013) Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. Mol Cell 49, 172-85
23142078   Curated Info

10

Wang H, et al. (2011) Convergence of the mammalian target of rapamycin complex 1- and glycogen synthase kinase 3-β-signaling pathways regulates the innate inflammatory response. J Immunol 186, 5217-26
21422248   Curated Info

11

Keshwani MM, Gao X, Harris TK (2009) Mechanism of PDK1-catalyzed Thr-229 phosphorylation of the S6K1 protein kinase. J Biol Chem 284, 22611-24
19570988   Curated Info

12

Keshwani MM, Harris TK (2008) Kinetic mechanism of fully activated S6K1 protein kinase. J Biol Chem 283, 11972-80
18326039   Curated Info

13

Yau CY, Wheeler JJ, Sutton KL, Hedley DW (2005) Inhibition of integrin-linked kinase by a selective small molecule inhibitor, QLT0254, inhibits the PI3K/PKB/mTOR, Stat3, and FKHR pathways and tumor growth, and enhances gemcitabine-induced apoptosis in human orthotopic primary pancreatic cancer xenografts. Cancer Res 65, 1497-504
15735038   Curated Info