Ser2548

Site Information
TRFGRKLsIIRGIVE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 40422837

In vivo Characterization
Methods used to characterize site in vivo:	[32P] ATP in vitro ( 1 ) , mass spectrometry ( 1 , 2 , 3 ) , mass spectrometry (in vitro) ( 1 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 3 ) , HEK293T (epithelial) ( 1 ) , LCL (lymphoblastoid) ( 2 ) , SF9 ( 1 , 2 )

Downstream Regulation
Effects of modification on Huntingtin:	protein degradation ( 1 )

References
1	Lee Y, et al. (2022) Huntingtin turnover: modulation of huntingtin degradation by cAMP-dependent protein kinase A (PKA) phosphorylation of C-HEAT domain Ser2550. Hum Mol Genet 35908190 Curated Info
2	Jung T, et al. (2020) The Polyglutamine Expansion at the N-Terminal of Huntingtin Protein Modulates the Dynamic Configuration and Phosphorylation of the C-Terminal HEAT Domain. Structure 28, 1035-1050.e8 32668197 Curated Info
3	Huang B, et al. (2015) Scalable production in human cells and biochemical characterization of full-length normal and mutant huntingtin. PLoS One 10, e0121055 25799558 Curated Info