Ser522
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Home > Phosphorylation Site Page: > Ser522  -  CRMP-2 (rat)

Site Information
PASsAKTsPAKQQAP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448082

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 8 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 5 , 6 ) , mutation of modification site ( 1 , 2 , 3 , 7 ) , phospho-antibody ( 3 , 4 , 8 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
breast cancer ( 3 ) , neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:
'neuron, cortical' ( 4 ) , 293 (epithelial) ( 1 , 4 ) , BCEC4 (endothelial) ( 5 ) , brain ( 8 ) , CAD (neuron) ( 1 ) , MDA-MB-231 (breast cell) ( 3 ) , neuron-dorsal root ganglia ( 1 ) , neuron-spinal cord ( 7 ) , REF (fibroblast) ( 2 ) , SH-SY5Y (neural crest) ( 4 )

Upstream Regulation
Regulatory protein:
PIN1 (human) ( 4 )
Putative in vivo kinases:
CDK5 (human) ( 4 ) , GSK3A (human) ( 2 )
Kinases, in vitro:
CDK5 (human) ( 4 , 7 )
Phosphatases, in vitro:
PPP1CA (human) ( 4 )
Treatments:
alsterpaullone ( 4 ) , AR-A014418 ( 2 ) , BAPTA-AM ( 4 ) , calyculin_A ( 4 ) , ciclosporin ( 4 ) , CT-99021 ( 4 ) , cypermethrin ( 4 ) , EGTA ( 4 , 8 ) , glycerol_phosphate ( 8 ) , IGF-1 ( 4 ) , KFl ( 8 ) , microcystin-LR ( 8 ) , okadaic_acid ( 4 , 8 ) , purvalanol ( 4 ) , sodium_phosphate ( 8 ) , sodium_pyrophosphate ( 8 ) , vanadate ( 8 )

Downstream Regulation
Effects of modification on CRMP-2:
intracellular localization ( 1 ) , molecular association, regulation ( 2 ) , sumoylation ( 1 )
Effects of modification on biological processes:
cell growth, altered ( 7 ) , endocytosis, induced ( 1 )
Inhibit interaction with:
ROCK2 (rat) ( 2 )

References 

1

Dustrude ET, et al. (2016) Hierarchical CRMP2 posttranslational modifications control NaV1.7 function. Proc Natl Acad Sci U S A 113, E8443-E8452
27940916   Curated Info

2

Morgan-Fisher M, Couchman JR, Yoneda A (2013) Phosphorylation and mRNA Splicing of Collapsin Response Mediator Protein-2 Determine Inhibition of Rho-associated Protein Kinase (ROCK) II Function in Carcinoma Cell Migration and Invasion. J Biol Chem 288, 31229-40
24036111   Curated Info

3

Yoneda A, et al. (2012) A Collapsin Response Mediator Protein 2 Isoform Controls Myosin II-Mediated Cell Migration and Matrix Assembly by Trapping ROCK II. Mol Cell Biol 32, 1788-804
22431514   Curated Info

4

Cole AR, et al. (2008) Relative Resistance of Cdk5-phosphorylated CRMP2 to Dephosphorylation. J Biol Chem 283, 18227-18237
18460467   Curated Info

5

Klemm C, et al. (2006) Evaluation of the titanium dioxide approach for MS analysis of phosphopeptides. J Mass Spectrom 41, 1623-32
17089331   Curated Info

6

Cole AR, et al. (2004) GSK-3 phosphorylation of the Alzheimer epitope within collapsin response mediator proteins regulates axon elongation in primary neurons. J Biol Chem 279, 50176-80
15466863   Curated Info

7

Brown M, et al. (2004) Alpha2-chimaerin, cyclin-dependent Kinase 5/p35, and its target collapsin response mediator protein-2 are essential components in semaphorin 3A-induced growth-cone collapse. J Neurosci 24, 8994-9004
15483118   Curated Info

8

Gu Y, Hamajima N, Ihara Y (2000) Neurofibrillary tangle-associated collapsin response mediator protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and Ser-522. Biochemistry 39, 4267-75
10757975   Curated Info