Lys170

Site Information
GRLCTVAkARGLRAC SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 40212801

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 2 ) , HEp-2 (squamous) ( 1 ) , Jurkat (T lymphocyte) ( 3 ) , MEF (fibroblast) ( 2 )

Downstream Regulation
Effects of modification on SIRT6:	protein stabilization ( 2 )
Effects of modification on biological processes:	DNA repair, induced ( 2 ) , transcription, inhibited ( 2 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Ronnebaum SM, Wu Y, McDonough H, Patterson C (2013) The ubiquitin ligase CHIP prevents SirT6 degradation through noncanonical ubiquitination. Mol Cell Biol 33, 4461-72 24043303 Curated Info
3	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info