Tyr684
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Home > Phosphorylation Site Page: > Tyr684  -  TrkA (rat)

Site Information
DIySTDyyRVGGRTM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447764

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 ) , mutation of modification site ( 2 , 4 , 5 , 6 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 ) , western blotting ( 6 )
Disease tissue studied:
adrenal cancer ( 6 ) , pheochromocytoma ( 6 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 5 ) , 'neuron, sympathetic' ( 3 ) , COS (fibroblast) ( 5 , 6 ) , neuron-'brain, hippocampus' ( 2 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 6 ) , PC-12 (chromaffin) ( 1 , 2 , 3 , 5 ) , SH-SY5Y (neural crest) ( 4 )

Upstream Regulation
Putative upstream phosphatases:
SHP-1 (human) ( 3 )
Treatments:
neurotrophin-3 ( 5 ) , NGF ( 3 , 4 , 5 , 6 )

Downstream Regulation
Effects of modification on TrkA:
enzymatic activity, induced ( 2 , 6 ) , molecular association, regulation ( 2 )
Effects of modification on biological processes:
cell cycle regulation ( 4 ) , cell growth, altered ( 4 ) , cytoskeletal reorganization ( 6 )
Induce interaction with:
DNAJA3 (mouse) ( 2 )

References 

1

Stokes M (2007) CST Curation Set: 3524; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Liu HY, et al. (2005) Human tumorous imaginal disc 1 (TID1) associates with Trk receptor tyrosine kinases and regulates neurite outgrowth in nnr5-TrkA cells. J Biol Chem 280, 19461-71
15753086   Curated Info

3

Marsh HN, et al. (2003) SHP-1 negatively regulates neuronal survival by functioning as a TrkA phosphatase. J Cell Biol 163, 999-1010
14662744   Curated Info

4

Gryz EA, Meakin SO (2003) Acidic substitution of the activation loop tyrosines in TrkA supports nerve growth factor-dependent, but not nerve growth factor-independent, differentiation and cell cycle arrest in the human neuroblastoma cell line, SY5Y. Oncogene 22, 8774-85
14647472   Curated Info

5

Liu HY, Meakin SO (2002) ShcB and ShcC activation by the Trk family of receptor tyrosine kinases. J Biol Chem 277, 26046-56
12006576   Curated Info

6

Gryz EA, Meakin SO (2000) Acidic substitution of the activation loop tyrosines in TrkA supports nerve growth factor-independent cell survival and neuronal differentiation. Oncogene 19, 417-30
10656690   Curated Info