Tyr499
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Home > Phosphorylation Site Page: > Tyr499  -  TrkA (rat)

Site Information
HIMENPQyFSDTCVH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447762
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 ) , mutation of modification site ( 5 , 9 ) , phospho-antibody ( 4 , 5 , 6 , 7 , 8 ) , western blotting ( 6 , 8 )
Disease tissue studied:
adrenal cancer ( 6 , 8 ) , pheochromocytoma ( 6 , 8 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 5 ) , COS (fibroblast) ( 5 ) , neuron:synaptosome-brain ( 7 ) , PC-12 (chromaffin) ( 2 , 3 , 4 , 5 , 6 , 8 , 9 )

Upstream Regulation
Regulatory protein:
SH2-B-beta (rat) ( 8 )
Treatments:
neurotrophin-3 ( 5 ) , NGF ( 5 , 6 , 8 ) , NS_1231 ( 6 ) , PP2 ( 4 ) , SU6656 ( 4 ) , TeTx ( 7 )

Downstream Regulation
Effects of modification on TrkA:
enzymatic activity, induced ( 8 ) , molecular association, regulation ( 5 )
Effects of modification on biological processes:
cell differentiation, altered ( 8 ) , cell growth, altered ( 9 )
Induce interaction with:
SHC2 (human) ( 5 ) , SHC3 (rat) ( 5 )

References 

1

Triaca V, et al. (2020) hNGF Peptides Elicit the NGF-TrkA Signalling Pathway in Cholinergic Neurons and Retain Full Neurotrophic Activity in the DRG Assay. Biomolecules
32024191   Curated Info

2

Stokes M (2007) CST Curation Set: 3524; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Stokes M (2007) CST Curation Set: 3525; Year: 2007; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Obara Y, Labudda K, Dillon TJ, Stork PJ (2004) PKA phosphorylation of Src mediates Rap1 activation in NGF and cAMP signaling in PC12 cells. J Cell Sci 117, 6085-94
15546918   Curated Info

5

Liu HY, Meakin SO (2002) ShcB and ShcC activation by the Trk family of receptor tyrosine kinases. J Biol Chem 277, 26046-56
12006576   Curated Info

6

Dagø L, et al. (2002) NS 1231, a novel compound with neurotrophic-like effects in vitro and in vivo. J Neurochem 81, 17-24
12067229   Curated Info

7

Gil C, Chaib-Oukadour I, Blasi J, Aguilera J (2001) HC fragment (C-terminal portion of the heavy chain) of tetanus toxin activates protein kinase C isoforms and phosphoproteins involved in signal transduction. Biochem J 356, 97-103
11336640   Curated Info

8

Qian X, Ginty DD (2001) SH2-B and APS are multimeric adapters that augment TrkA signaling. Mol Cell Biol 21, 1613-20
11238898   Curated Info

9

Obermeier A, et al. (1994) Neuronal differentiation signals are controlled by nerve growth factor receptor/Trk binding sites for SHC and PLC gamma. EMBO J 13, 1585-90
8156997   Curated Info