Ser400
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser400  -  Cot (mouse)

Site Information
EDQPRCQsLDSALFE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447712

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 3 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 2 , 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 , 3 ) , HEK293T (epithelial) ( 1 ) , macrophage-bone marrow ( 2 ) , macrophage-bone marrow [Cot (mouse)] ( 3 ) , macrophage-bone marrow [NFkB-p105 (human)] ( 3 ) , macrophage-bone marrow [NFkB-p105 (mouse)] ( 3 ) , PK-15 (epithelial) ( 1 ) , RAW 264 (macrophage) ( 3 )

Upstream Regulation
Regulatory protein:
Cot (mouse) ( 3 ) , IKKB (human) ( 2 ) , NFkB-p105 (mouse) ( 3 )
Treatments:
IL-1b ( 2 ) , LPS ( 3 ) , LY294002 ( 3 ) , wortmannin ( 3 )

Downstream Regulation
Effects of modification on Cot:
activity, induced ( 3 ) , molecular association, regulation ( 2 ) , phosphorylation ( 2 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 )
Induce interaction with:
14-3-3 gamma (human) ( 2 ) , ERK2 (mouse) ( 3 )

References 

1

Hao J, et al. (2020) Foot-and-Mouth Disease Virus Capsid Protein VP1 Antagonizes TPL2-Mediated Activation of the IRF3/IFN-β Signaling Pathway to Facilitate the Virus Replication. Front Immunol 11, 580334
33488582   Curated Info

2

Ben-Addi A, et al. (2014) IκB kinase-induced interaction of TPL-2 kinase with 14-3-3 is essential for Toll-like receptor activation of ERK-1 and -2 MAP kinases. Proc Natl Acad Sci U S A 111, E2394-403
24912162   Curated Info

3

Robinson MJ, et al. (2007) Phosphorylation of TPL-2 on serine 400 is essential for lipopolysaccharide activation of extracellular signal-regulated kinase in macrophages. Mol Cell Biol 27, 7355-64
17709378   Curated Info