Thr1465
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Home > Phosphorylation Site Page: > Thr1465  -  TSC2 (mouse)

Site Information
GLRPRGYtISDSAPS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448546

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 5 ) , phospho-antibody ( 3 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , western blotting ( 3 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 , 5 ) , 'muscle, skeletal' ( 14 ) , 'stem, embryonic' ( 14 ) , 293 (epithelial) ( 6 , 10 , 15 ) , 3T3-L1 (fibroblast) ( 11 ) , adipose tissue ( 14 ) , beta-pancreas [INSR (mouse), transgenic] ( 9 ) , fibroblast-embryo ( 6 ) , heart ( 3 , 14 ) , hepatocyte-liver [TSC1 (mouse), homozygous knockout] ( 8 ) , liver ( 14 ) , MEF (fibroblast) ( 10 , 12 , 16 , 17 , 18 ) , MEF (fibroblast) [GADD34 (human)] ( 15 ) , MEF (fibroblast) [Sin1 (mouse), homozygous knockout] ( 13 ) , myoblast ( 19 )

Upstream Regulation
Regulatory protein:
GADD34 (human) ( 15 ) , LST8 (mouse) ( 16 ) , PCTP (human) ( 6 ) , PDK1 (mouse) ( 14 ) , RICTOR (human) ( 10 , 12 ) , RICTOR (mouse) ( 10 , 16 ) , Sin1 (mouse) ( 17 ) , THEM2 (human) ( 6 )
Treatments:
exercise ( 3 ) , glucose ( 9 ) , GSK2334470 ( 2 ) , insulin ( 2 , 5 , 8 , 9 , 11 , 12 , 14 , 16 , 17 ) , LY294002 ( 2 , 5 ) , MK-2206 ( 2 , 5 ) , PDGF ( 11 ) , rapamycin ( 8 , 19 ) , serum ( 10 , 17 ) , serum_starvation ( 17 ) , TNF ( 18 ) , U0126 ( 9 ) , wortmannin ( 9 )

References 

1

Fabrik I, et al. (2018) The Early Dendritic Cell Signaling Induced by Virulent Francisella tularensis Strain Occurs in Phases and Involves the Activation of Extracellular Signal-Regulated Kinases (ERKs) and p38 In the Later Stage. Mol Cell Proteomics 17, 81-94
29046388   Curated Info

2

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

3

Sturgeon K, et al. (2015) Moderate-intensity treadmill exercise training decreases murine cardiomyocyte cross-sectional area. Physiol Rep 3
25991723   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Ersoy BA, et al. (2013) Phosphatidylcholine transfer protein interacts with thioesterase superfamily member 2 to attenuate insulin signaling. Sci Signal 6, ra64
23901139   Curated Info

7

Lu Y, et al. (2011) Kinome siRNA-phosphoproteomic screen identifies networks regulating AKT signaling. Oncogene 30, 4567-77
21666717   Curated Info

8

Yecies JL, et al. (2011) Akt Stimulates Hepatic SREBP1c and Lipogenesis through Parallel mTORC1-Dependent and Independent Pathways. Cell Metab 14, 21-32
21723501   Curated Info

9

Bartolomé A, Guillén C, Benito M (2010) Role of the TSC1-TSC2 complex in the integration of insulin and glucose signaling involved in pancreatic beta-cell proliferation. Endocrinology 151, 3084-94
20427478   Curated Info

10

Treins C, et al. (2010) Rictor is a novel target of p70 S6 kinase-1. Oncogene 29, 1003-16
19935711   Curated Info

11

Ng Y, et al. (2010) Cluster analysis of insulin action in adipocytes reveals a key role for Akt at the plasma membrane. J Biol Chem 285, 2245-57
19897488   Curated Info

12

Dibble CC, Asara JM, Manning BD (2009) Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol 29, 5657-70
19720745   Curated Info

13

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

14

Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72
18347057   Curated Info

15

Minami K, et al. (2007) Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway. J Virol 81, 11106-15
17670836   Curated Info

16

Guertin DA, et al. (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11, 859-71
17141160   Curated Info

17

Jacinto E, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127, 125-37
16962653   Curated Info

18

Ghosh S, et al. (2006) Essential role of tuberous sclerosis genes TSC1 and TSC2 in NF-kappaB activation and cell survival. Cancer Cell 10, 215-26
16959613   Curated Info

19

Ohanna M, et al. (2005) Atrophy of S6K1(-/-) skeletal muscle cells reveals distinct mTOR effectors for cell cycle and size control. Nat Cell Biol 7, 286-94
15723049   Curated Info