Ser939
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Home > Phosphorylation Site Page: > Ser939  -  TSC2 (mouse)

Site Information
SFRARSTsLNERPKs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448501

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ) , phospho-antibody ( 10 , 18 , 19 , 20 ) , western blotting ( 10 , 18 , 19 , 20 )
Disease tissue studied:
breast cancer ( 18 ) , melanoma skin cancer ( 17 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 , 4 , 5 ) , 'fat, brown' ( 9 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 16 ) , 32Dcl3 (myeloid) ( 16 ) , beta-pancreas [INSR (mouse), transgenic] ( 10 ) , brain ( 9 ) , fibroblast-lung ( 12 , 13 , 14 , 15 ) , heart ( 6 ) , kidney ( 9 ) , liver ( 1 , 8 ) , liver [leptin (mouse), homozygous knockout] ( 8 ) , lung ( 9 ) , macrophage-bone marrow ( 11 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 11 ) , macrophage-peritoneum ( 7 ) , mammary gland ( 18 ) , MEF (fibroblast) ( 19 , 20 ) , pancreas ( 9 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 17 ) , spleen ( 9 )

Upstream Regulation
Regulatory protein:
LST8 (mouse) ( 19 ) , RICTOR (mouse) ( 19 ) , Sin1 (mouse) ( 20 )
Putative in vivo kinases:
Akt1 (mouse) ( 18 )
Treatments:
glucose ( 10 ) , insulin ( 4 , 5 , 10 , 19 , 20 ) , LY294002 ( 4 ) , MK-2206 ( 4 , 5 ) , serum ( 20 ) , serum_starvation ( 20 ) , U0126 ( 10 ) , wortmannin ( 10 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

4

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Bartolomé A, Guillén C, Benito M (2010) Role of the TSC1-TSC2 complex in the integration of insulin and glucose signaling involved in pancreatic beta-cell proliferation. Endocrinology 151, 3084-94
20427478   Curated Info

11

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

12

Guo A (2010) CST Curation Set: 9813; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Guo A (2010) CST Curation Set: 9810; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

14

Guo A (2010) CST Curation Set: 9811; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

15

Guo A (2010) CST Curation Set: 9812; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

16

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

17

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

18

Ju X, et al. (2007) Akt1 governs breast cancer progression in vivo. Proc Natl Acad Sci U S A 104, 7438-43
17460049   Curated Info

19

Guertin DA, et al. (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11, 859-71
17141160   Curated Info

20

Jacinto E, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127, 125-37
16962653   Curated Info