Ser8
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.5
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser8  -  TH (rat)

Site Information
MPTPSAPsPQPKGFR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448543

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 6 ) , mass spectrometry ( 1 ) , peptide sequencing ( 5 , 6 ) , phosphoamino acid analysis ( 5 , 6 ) , phosphopeptide mapping ( 5 , 6 )
Disease tissue studied:
adrenal cancer ( 6 ) , pheochromocytoma ( 6 )
Relevant cell line - cell type - tissue:
PC-12 (chromaffin) [TrkA (rat), transfection] ( 1 ) , PC-12 (chromaffin) ( 1 , 6 )

Upstream Regulation
Kinases, in vitro:
ERK2 (human) ( 2 ) , ERK2 (rat) ( 4 )
Treatments:
colforsin ( 5 ) , depolarization ( 5 ) , EGF ( 6 ) , electrical_stimulation ( 5 ) , okadaic_acid ( 6 ) , PDGF ( 1 ) , phorbol_ester ( 5 )

Downstream Regulation
Effects of modification on TH:
protein stabilization ( 3 )

References 

1

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

2

Royo M, Colette Daubner S (2006) Kinetics of regulatory serine variants of tyrosine hydroxylase with cyclic AMP-dependent protein kinase and extracellular signal-regulated protein kinase 2. Biochim Biophys Acta 1764, 786-92
16503426   Curated Info

3

Royo M, Fitzpatrick PF, Daubner SC (2005) Mutation of regulatory serines of rat tyrosine hydroxylase to glutamate: effects on enzyme stability and activity. Arch Biochem Biophys 434, 266-74
15639226   Curated Info

4

Royo M, Daubner SC, Fitzpatrick PF (2004) Specificity of the MAP kinase ERK2 for phosphorylation of tyrosine hydroxylase. Arch Biochem Biophys 423, 247-52
15001389   Curated Info

5

Haycock JW, Haycock DA (1991) Tyrosine hydroxylase in rat brain dopaminergic nerve terminals. Multiple-site phosphorylation in vivo and in synaptosomes. J Biol Chem 266, 5650-7
1672315   Curated Info

6

Haycock JW (1990) Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40. J Biol Chem 265, 11682-91
1973163   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.