Ser456

Site Information
GFPtIyFsPANkKLN SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 3963114

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 ) , mass spectrometry (in vitro) ( 4 )
Disease tissue studied:	lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:	HeLa (cervical) ( 2 , 3 ) , PC9 (pulmonary) ( 1 ) , PC9-IR (pulmonary) ( 1 )

Upstream Regulation
Kinases, in vitro:	CDK1 (human) ( 4 )

References
1	Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622 25814448 Curated Info
2	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
3	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
4	Blethrow JD, Glavy JS, Morgan DO, Shokat KM (2008) Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. Proc Natl Acad Sci U S A 105, 1442-7 18234856 Curated Info