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Site Information |
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tVNQsLLsPLVLEVD SwissProt Entrez-Gene |
Blast this site against: NCBI SwissProt PDB |
Site Group ID: 448539 |
In vivo Characterization | |
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Methods used to characterize site in vivo: | |
Disease tissue studied: | |
Relevant cell line - cell type - tissue: |
Upstream Regulation | |
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Regulatory protein: | |
Putative in vivo kinases: | |
Kinases, in vitro: | |
Putative upstream phosphatases: | |
Treatments: |
Downstream Regulation | |
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Effects of modification on K8: | |
Effects of modification on biological processes: | |
Induce interaction with: |
Disease / Diagnostics Relevance | |
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Relevant diseases: |
References | |
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Xia B, et al. (2020) Leukamenin E Induces K8/18 Phosphorylation and Blocks the Assembly of Keratin Filament Networks Through ERK Activation. Int J Mol Sci 21
32365802 Curated Info |
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Lee SY, et al. (2019) Keratins regulate Hsp70-mediated nuclear localization of p38 Mitogen-activated protein kinase. J Cell Sci
31427430 Curated Info |
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Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782 Curated Info |
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Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275 Curated Info |
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Guldiken N, et al. (2015) Human keratin 8 variants promote mouse acetaminophen hepatotoxicity coupled with c-jun amino-terminal kinase activation and protein adduct formation. Hepatology 62, 876-86
25963979 Curated Info |
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Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448 Curated Info |
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Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569 Curated Info |
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Snider NT, et al. (2013) Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol 200, 241-7
23358244 Curated Info |
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Park MK, et al. (2012) Novel involvement of leukotriene B₄ receptor 2 through ERK activation by PP2A down-regulation in leukotriene B₄-induced keratin phosphorylation and reorganization of pancreatic cancer cells. Biochim Biophys Acta 1823, 2120-9
23017243 Curated Info |
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Moritz A (2011) CST Curation Set: 11935; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info |
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Moritz A (2011) CST Curation Set: 11936; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
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Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994 Curated Info |
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Alam H, et al. (2011) Loss of Keratin 8 Phosphorylation Leads to Increased Tumor Progression and Correlates with Clinico-Pathological Parameters of OSCC Patients. PLoS One 6, e27767
22114688 Curated Info |
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Menon MB, et al. (2010) p38 MAP kinase and MAPKAP kinases MK2/3 cooperatively phosphorylate epithelial keratins. J Biol Chem 285, 33242-51
20724476 Curated Info |
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McIntosh PB, et al. (2010) E1--E4-mediated keratin phosphorylation and ubiquitylation: a mechanism for keratin depletion in HPV16-infected epithelium. J Cell Sci 123, 2810-22
20663917 Curated Info |
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Tucker M (2010) CST Curation Set: 9842; Year: 2010; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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Tucker M (2010) CST Curation Set: 9836; Year: 2010; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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Moritz A (2010) CST Curation Set: 9775; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info |
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Moritz A (2010) CST Curation Set: 9234; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info |
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Moritz A (2010) CST Curation Set: 9236; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info |
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Kakehashi A, et al. (2010) Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions. Toxicol Appl Pharmacol 242, 47-55
19796649 Curated Info |
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Flitney EW, Kuczmarski ER, Adam SA, Goldman RD (2009) Insights into the mechanical properties of epithelial cells: the effects of shear stress on the assembly and remodeling of keratin intermediate filaments. FASEB J 23, 2110-9
19246484 Curated Info |
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Mizuuchi E, Semba S, Kodama Y, Yokozaki H (2009) Down-modulation of keratin 8 phosphorylation levels by PRL-3 contributes to colorectal carcinoma progression. Int J Cancer 124, 1802-10
19115206 Curated Info |
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Jaitovich A, et al. (2008) Ubiquitin-proteasome-mediated degradation of keratin intermediate filaments in mechanically stimulated A549 cells. J Biol Chem 283, 25348-55
18617517 Curated Info |
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Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976 Curated Info |
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Zhou J (2008) CST Curation Set: 4778; Year: 2008; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-ATM/ATR Substrate Motif (pS/pTQG) Antibody Cat#: 5074, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQG) Immunoaffinity Beads Cat#: 1993
Curated Info |
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Moritz A (2008) CST Curation Set: 4208; Year: 2008; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info |
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Wöll S, Windoffer R, Leube RE (2007) p38 MAPK-dependent shaping of the keratin cytoskeleton in cultured cells. J Cell Biol 177, 795-807
17535969 Curated Info |
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Tao GZ, et al. (2006) Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner. J Cell Sci 119, 1425-32
16554440 Curated Info |
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Tao GZ, et al. (2006) Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies. Exp Cell Res 312, 411-22
16343483 Curated Info |
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Scott GK, et al. (2005) Vitamin K3 (menadione)-induced oncosis associated with keratin 8 phosphorylation and histone H3 arylation. Mol Pharmacol 68, 606-15
15939799 Curated Info |
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Ridge KM, et al. (2005) Keratin 8 phosphorylation by protein kinase C delta regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells. J Biol Chem 280, 30400-5
15972820 Curated Info |
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Chen YL, et al. (2005) Requirement for ERK activation in acetone extract identified from Bupleurum scorzonerifolium induced A549 tumor cell apoptosis and keratin 8 phosphorylation. Life Sci 76, 2409-20
15763073 Curated Info |
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Gires O, et al. (2005) Cytokeratin 8 associates with the external leaflet of plasma membranes in tumour cells. Biochem Biophys Res Commun 328, 1154-62
15707998 Curated Info |
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Toivola DM, et al. (2004) Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease. Hepatology 40, 459-66
15368451 Curated Info |
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He T, et al. (2002) The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J Biol Chem 277, 10767-74
11781324 Curated Info |
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Ku NO, Azhar S, Omary MB (2002) Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation. J Biol Chem 277, 10775-82
11788583 Curated Info |
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Stumptner C, et al. (2000) Hepatocyte cytokeratins are hyperphosphorylated at multiple sites in human alcoholic hepatitis and in a mallory body mouse model. Am J Pathol 156, 77-90
10623656 Curated Info |
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Liao J, Ku NO, Omary MB (1997) Stress, apoptosis, and mitosis induce phosphorylation of human keratin 8 at Ser-73 in tissues and cultured cells. J Biol Chem 272, 17565-73
9211903 Curated Info |