Thr309
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Home > Phosphorylation Site Page: > Thr309  -  Akt2 (mouse)

Site Information
sDGAtMKtFCGtPEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448537

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 ) , phospho-antibody ( 4 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 4 ) , brain ( 3 ) , embryo ( 3 ) , heart ( 2 ) , liver ( 3 ) , SF9 ( 4 )

Upstream Regulation
Putative upstream phosphatases:
PTEN (mouse) ( 4 )
Treatments:
insulin ( 1 , 4 ) , LY294002 ( 1 ) , MK-2206 ( 1 )

References 

1

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

2

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

3

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

4

Ono H, et al. (2001) Regulation of phosphoinositide metabolism, Akt phosphorylation, and glucose transport by PTEN (phosphatase and tensin homolog deleted on chromosome 10) in 3T3-L1 adipocytes. Mol Endocrinol 15, 1411-22
11463863   Curated Info