Thr161
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Home > Phosphorylation Site Page: > Thr161  -  CDK1 (mouse)

Site Information
GIPIRVytHEVVTLW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447609

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 5 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ) , mutation of modification site ( 18 ) , phospho-antibody ( 2 , 4 , 9 , 18 ) , western blotting ( 2 , 4 , 9 , 18 )
Relevant cell line - cell type - tissue:
'B lymphocyte, precursor' ( 18 ) , 'fat, brown' ( 8 ) , 'stem, embryonic' ( 11 , 12 , 13 , 14 , 15 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 16 ) , 32Dcl3 (myeloid) ( 16 ) , C2C12 (myoblast) ( 17 ) , E14tg2a ('stem, embryonic') ( 9 ) , heart ( 8 ) , HeLa_BI (cervical) ( 7 ) , HeLa_Hesp (cervical) ( 7 ) , HeLa_NOC (cervical) ( 7 ) , kidney ( 8 ) , liver ( 8 ) , lung ( 8 ) , MEF (fibroblast) ( 4 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 6 ) , mpkCCD (renal) ( 10 ) , R1 ('stem, embryonic') ( 9 ) , RAW 264 (macrophage) ( 2 ) , spleen ( 8 ) , testis ( 8 ) , thymocyte ( 18 )

Upstream Regulation
Regulatory protein:
Akt1 (mouse) ( 4 ) , Akt2 (mouse) ( 4 ) , CCNH (mouse) ( 9 )
Treatments:
IFN-gamma ( 2 ) , IL-3_withdrawal ( 18 ) , LPS ( 2 ) , serum ( 4 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Xu K, Harrison RE (2015) Down-regulation of Stathmin Is Required for the Phenotypic Changes and Classical Activation of Macrophages. J Biol Chem 290, 19245-60
26082487   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Nogueira V, et al. (2012) Akt-dependent Skp2 mRNA translation is required for exiting contact inhibition, oncogenesis, and adipogenesis. EMBO J 31, 1134-46
22307088   Curated Info

5

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

6

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

7

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Patel SA, Simon MC (2010) Functional analysis of the Cdk7.cyclin H.Mat1 complex in mouse embryonic stem cells and embryos. J Biol Chem 285, 15587-98
20231280   Curated Info

10

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

11

Tucker M (2010) CST Curation Set: 9080; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

12

Tucker M (2010) CST Curation Set: 9078; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

13

Tucker M (2009) CST Curation Set: 8601; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

14

Tucker M (2009) CST Curation Set: 8604; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

15

Tucker M (2009) CST Curation Set: 8607; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

16

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

17

Possemato A (2008) CST Curation Set: 5030; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

18

Khaled AR, et al. (2005) Cytokine-driven cell cycling is mediated through Cdc25A. J Cell Biol 169, 755-63
15928203   Curated Info