Ser70
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Home > Phosphorylation Site Page: > Ser70  -  Bcl-2 (mouse)

Site Information
RDMAARtsPLRPLVA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448395

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 , 7 , 8 ) , immunoprecipitation ( 8 ) , mass spectrometry ( 2 ) , mutation of modification site ( 3 , 5 , 6 , 7 , 8 ) , phospho-antibody ( 1 , 3 , 4 ) , western blotting ( 1 , 3 , 4 )
Disease tissue studied:
chondrosarcoma ( 1 ) , leukemia ( 6 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 2 ) , brain ( 3 ) , chondrocyte ( 1 ) , FDCP-1 (myeloid) ( 6 ) , NCI-H157 (pulmonary) ( 5 ) , NSF/N1.H7 (myeloid) ( 5 , 6 , 7 , 8 ) , RCS (chondrocyte) ( 1 ) , retina ( 3 )

Upstream Regulation
Regulatory protein:
FGF18 (mouse) ( 1 )
Putative in vivo kinases:
CDK5 (mouse) ( 3 ) , ERK1 (mouse) ( 7 ) , ERK2 (mouse) ( 7 ) , JNK1 (human) ( 6 )
Kinases, in vitro:
CDK5 (mouse) ( 3 ) , ERK1 (human) ( 7 ) , ERK2 (human) ( 7 ) , JNK1 (human) ( 6 )
Putative upstream phosphatases:
PPP2CA (mouse) ( 4 )
Phosphatases, in vitro:
PPP2CA (mouse) ( 4 )
Treatments:
anisomycin ( 6 ) , aurintricarboxylic_acid ( 7 ) , bryostatin_1 ( 7 , 8 ) , C2-ceramide ( 4 ) , etoposide ( 4 , 8 ) , IL-3 ( 6 , 8 ) , IL-3_withdrawal ( 8 ) , lithium ( 4 ) , okadaic_acid ( 4 , 6 ) , PD98059 ( 7 ) , SP600125 ( 1 ) , staurosporine ( 7 )

Downstream Regulation
Effects of modification on Bcl-2:
molecular association, regulation ( 7 ) , protein stabilization ( 5 )
Effects of modification on biological processes:
apoptosis, inhibited ( 3 , 5 , 6 , 7 , 8 ) , cell cycle regulation ( 5 )
Induce interaction with:
BAX (mouse) ( 7 )

References 

1

Cinque L, et al. (2015) FGF signalling regulates bone growth through autophagy. Nature 528, 272-5
26595272   Curated Info

2

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
19737024   Curated Info

3

Cheung ZH, Gong K, Ip NY (2008) Cyclin-dependent kinase 5 supports neuronal survival through phosphorylation of Bcl-2. J Neurosci 28, 4872-7
18463240   Curated Info

4

Chen CL, et al. (2006) Lithium inhibits ceramide- and etoposide-induced protein phosphatase 2A methylation, Bcl-2 dephosphorylation, caspase-2 activation, and apoptosis. Mol Pharmacol 70, 510-7
16682503   Curated Info

5

Deng X, Gao F, Flagg T, May WS (2004) Mono- and multisite phosphorylation enhances Bcl2's antiapoptotic function and inhibition of cell cycle entry functions. Proc Natl Acad Sci U S A 101, 153-8
14660795   Curated Info

6

Deng X, et al. (2001) Novel role for JNK as a stress-activated Bcl2 kinase. J Biol Chem 276, 23681-8
11323415   Curated Info

7

Deng X, Ruvolo P, Carr B, May WS (2000) Survival function of ERK1/2 as IL-3-activated, staurosporine-resistant Bcl2 kinases. Proc Natl Acad Sci U S A 97, 1578-83
10677502   Curated Info

8

Ito T, Deng X, Carr B, May WS (1997) Bcl-2 phosphorylation required for anti-apoptosis function. J Biol Chem 272, 11671-3
9115213   Curated Info