Ser108

Site Information
skLPLTRsHNNFVAI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448522

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 2 ) , mass spectrometry ( 1 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 13 , 15 , 16 , 17 , 19 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 38 , 39 , 40 , 41 , 42 , 43 , 45 ) , mutation of modification site ( 18 , 44 ) , phospho-antibody ( 2 , 14 ) , western blotting ( 14 )
Disease tissue studied:	breast cancer ( 8 , 14 , 17 ) , breast adenocarcinoma ( 14 ) , breast ductal carcinoma ( 8 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 8 , 14 ) , cervical cancer ( 34 ) , cervical adenocarcinoma ( 34 ) , leukemia ( 21 ) , acute myelogenous leukemia ( 21 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 16 ) , acute megakaryoblastic leukemia (M7) ( 16 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 16 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 16 ) , acute myeloblastic leukemia, without maturation (M1) ( 16 ) , hepatocellular carcinoma, surrounding tissue ( 32 ) , lung cancer ( 17 , 27 ) , non-small cell lung cancer ( 17 ) , B cell lymphoma ( 16 ) , non-Hodgkin's lymphoma ( 16 ) , neuroblastoma ( 1 , 15 ) , ovarian cancer ( 8 ) , multiple myeloma ( 16 , 31 ) , prostate cancer ( 33 ) , melanoma skin cancer ( 6 ) , rhabdomyosarcoma ( 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 29 ) , 293 (epithelial) ( 18 , 44 ) , 293E (epithelial) ( 23 ) , A498 (renal) ( 30 ) , AML-193 (monocyte) ( 16 ) , AU565 (breast cell) ( 14 ) , B lymphocyte-blood ( 31 ) , breast ( 3 , 8 ) , BT-20 (breast cell) ( 17 ) , BT-549 (breast cell) ( 17 ) , Calu 6 (pulmonary) ( 17 ) , CL1-0 (pulmonary) ( 27 ) , CL1-1 (pulmonary) ( 27 ) , CL1-2 (pulmonary) ( 27 ) , CL1-5 (pulmonary) ( 27 ) , CMK (megakaryoblast) ( 16 ) , CTS (myeloid) ( 16 ) , DG75 (B lymphocyte) ( 28 ) , DOHH2 ('B lymphocyte, precursor') ( 16 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 19 ) , Flp-In T-Rex-293 (epithelial) ( 19 ) , H2009 (pulmonary) ( 17 ) , H2077 (pulmonary) ( 17 ) , H2887 (pulmonary) ( 17 ) , H322M (pulmonary) ( 17 ) , HCC1359 (pulmonary) ( 17 ) , HCC1937 (breast cell) ( 17 ) , HCC2279 (pulmonary) ( 17 ) , HCC366 (pulmonary) ( 17 ) , HCC4006 (pulmonary) ( 17 ) , HCC78 (pulmonary) ( 17 ) , HCC827 (pulmonary) ( 17 ) , HCT116 (intestinal) ( 36 ) , HEL (erythroid) ( 16 ) , HeLa (cervical) ( 7 , 13 , 26 , 35 , 39 , 42 ) , HeLa S3 (cervical) ( 34 , 41 ) , hepatocyte-liver ( 32 ) , HUES-9 ('stem, embryonic') ( 25 ) , Jurkat (T lymphocyte) ( 11 , 22 , 24 , 40 ) , K562 (erythroid) ( 13 , 35 ) , Kasumi-1 (myeloid) ( 16 ) , KG-1 (myeloid) ( 16 , 21 ) , KPL-4 (breast cell) ( 14 ) , LCLC-103H (pulmonary) ( 17 ) , liver ( 10 ) , LNCaP (prostate cell) ( 33 ) , LOU-NH91 (squamous) ( 17 ) , MCF-7 (breast cell) ( 5 , 14 , 17 ) , MDA-IBC-3 (breast cell) ( 14 ) , MDA-MB-231 (breast cell) ( 14 , 17 ) , MDA-MB-435S (breast cell) ( 36 ) , MDA-MB-468 (breast cell) ( 14 , 17 ) , MV4-11 (macrophage) ( 16 , 36 ) , NB10 (neural crest) ( 15 ) , NCI-H1299 (pulmonary) ( 45 ) , NCI-H1568 (pulmonary) ( 17 ) , NCI-H157 (pulmonary) ( 17 ) , NCI-H1648 (pulmonary) ( 17 ) , NCI-H1666 (pulmonary) ( 17 ) , NCI-H2172 (pulmonary) ( 17 ) , NCI-H460 (pulmonary) ( 17 ) , NCI-H520 (squamous) ( 17 ) , NCI-H647 (pulmonary) ( 17 ) , NPC (neural crest) ( 15 ) , OPM-2 (plasma cell) ( 16 ) , ovary ( 8 ) , P31/FUJ (erythroid) ( 16 ) , PC9 (pulmonary) ( 17 ) , platelet-blood ( 43 ) , Rh30 ('muscle, skeletal') ( 2 ) , RL ('B lymphocyte, precursor') ( 16 ) , RPMI-8266 (plasma cell) ( 16 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 9 ) , SH-SY5Y (neural crest) ( 9 ) , SK-N-BE(2) (neural crest) ( 1 ) , SKBr3 (breast cell) ( 14 ) , SU-DHL-6 (B lymphocyte) ( 16 ) , SUM149 (breast cell) ( 14 ) , SUM159 (breast cell) ( 14 ) , SUM190 (breast cell) ( 14 ) , U266 (plasma cell) ( 16 ) , WM115 (melanocyte) ( 38 ) , WM239A (melanocyte) ( 6 )

Upstream Regulation
Kinases, in vitro:	AMPKA1 (human) ( 37 )
Treatments:	angiotensin_2 ( 29 ) , antibody ( 40 ) , cannabidiol ( 1 ) , dasatinib ( 35 ) , EGF ( 35 ) , ionizing_radiation ( 45 ) , ischemia ( 8 ) , Lys05 ( 45 ) , metformin ( 5 ) , nocodazole ( 34 ) , SB202190 ( 35 ) , U0126 ( 35 )

Downstream Regulation
Effects of modification on AMPKB1:	activity, induced ( 44 ) , molecular association, regulation ( 18 ) , phosphorylation ( 44 )

References
1	Guard SE, et al. (2022) Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell Lines. Mol Cell Proteomics, 100262 35753663 Curated Info
2	Dasgupta A, et al. (2020) Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma. Cancer Res 33168646 Curated Info
3	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
4	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
5	Sacco F, et al. (2016) Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling Networks Away from a Pro-growth State. Cell Syst 2, 159-71 27135362 Curated Info
6	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
7	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
8	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
9	Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76 24117733 Curated Info
10	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
11	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
12	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
13	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
14	Robertson FM, et al. (2013) Presence of anaplastic lymphoma kinase in inflammatory breast cancer. Springerplus 2, 497 24102046 Curated Info
15	DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513 24349301 Curated Info
16	Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37 23628362 Curated Info
17	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
18	Hawley SA, et al. (2012) The ancient drug salicylate directly activates AMP-activated protein kinase. Science 336, 918-22 22517326 Curated Info
19	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
20	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
21	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
22	Mulhern D (2011) CST Curation Set: 12711; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Curated Info
23	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
24	Guo A (2011) CST Curation Set: 11984; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Curated Info
25	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
26	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
27	Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97 20815410 Curated Info
28	Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72 20562096 Curated Info
29	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
30	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
31	Ge F, et al. (2010) Phosphoproteomic analysis of primary human multiple myeloma cells. J Proteomics 73, 1381-90 20230923 Curated Info
32	Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9 20166139 Curated Info
33	Chen L, Giorgianni F, Beranova-Giorgianni S (2010) Characterization of the phosphoproteome in LNCaP prostate cancer cells by in-gel isoelectric focusing and tandem mass spectrometry. J Proteome Res 9, 174-8 20044836 Curated Info
34	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
35	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
36	Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64 19369195 Curated Info
37	Tuerk RD, et al. (2009) Tracking and quantification of 32P-labeled phosphopeptides in liquid chromatography matrix-assisted laser desorption/ionization mass spectrometry. Anal Biochem 390, 141-8 19376078 Curated Info
38	Old WM, et al. (2009) Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Mol Cell 34, 115-31 19362540 Curated Info
39	Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8 19276368 Curated Info
40	Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46 19690332 Curated Info
41	Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48 18691976 Curated Info
42	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
43	Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34 18088087 Curated Info
44	Sanders MJ, et al. (2007) Defining the mechanism of activation of AMP-activated protein kinase by the small molecule A-769662, a member of the thienopyridone family. J Biol Chem 282, 32539-48 17728241 Curated Info
45	Ondrej M, et al. Lys05 - A Promising Autophagy Inhibitor in the Radiosensitization Battle: Phosphoproteomic Perspective. Cancer Genomics Proteomics 32576582 Curated Info