Ser474
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Home > Phosphorylation Site Page: > Ser474  -  Akt2 (mouse)

Site Information
RTHFPQFsysAsIRE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448520
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 4 , 5 , 7 , 8 ) , mutation of modification site ( 9 ) , phospho-antibody ( 6 , 9 , 10 ) , western blotting ( 6 , 9 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 , 3 ) , 'fat, brown' ( 5 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 10 ) , 3T3-L1 (fibroblast) ( 6 ) , brain ( 4 ) , embryo ( 4 ) , heart ( 7 ) , liver ( 4 , 8 ) , myoblast ( 9 ) , SF9 ( 10 )

Upstream Regulation
Putative upstream phosphatases:
PTEN (mouse) ( 10 )
Treatments:
dexamethasone ( 6 ) , insulin ( 1 , 3 , 6 , 10 ) , LY294002 ( 1 , 3 ) , MK-2206 ( 1 , 3 ) , serum_withdrawal ( 9 ) , Torin1 ( 1 )

References 

1

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

5

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

6

Ng Y, Ramm G, James DE (2010) Dissecting the mechanism of insulin resistance using a novel heterodimerization strategy to activate Akt. J Biol Chem 285, 5232-9
20022950   Curated Info

7

Guo A (2007) CST Curation Set: 3118; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

8

Guo A (2007) CST Curation Set: 3119; Year: 2007; Biosample/Treatment: tissue, liver/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y)p[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb Cat#: 9634, PTMScan(R) Phospho-PDK1 Docking Motif (F/YS*/T*F/Y) Immunoaffinity Beads Cat#: 1992
Curated Info

9

Gonzalez I, et al. (2004) Akt2, a novel functional link between p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase pathways in myogenesis. Mol Cell Biol 24, 3607-22
15082758   Curated Info

10

Ono H, et al. (2001) Regulation of phosphoinositide metabolism, Akt phosphorylation, and glucose transport by PTEN (phosphatase and tensin homolog deleted on chromosome 10) in 3T3-L1 adipocytes. Mol Endocrinol 15, 1411-22
11463863   Curated Info