Thr450
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Home > Phosphorylation Site Page: > Thr450  -  Akt1 (mouse)

Site Information
TAQMITItPPDQDDS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447857

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 1 ) , mutation of modification site ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 )
Disease tissue studied:
neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:
'neuron, cortical'-brain ( 1 ) , 293 (epithelial) ( 6 , 8 ) , HepG2 (hepatic) ( 8 ) , MEF (fibroblast) ( 2 , 4 , 5 , 6 ) , MEF (fibroblast) [Sin1 (mouse), homozygous knockout] ( 7 ) , myocyte-heart ( 3 ) , N1E-115 (neuron) ( 1 )

Upstream Regulation
Regulatory protein:
RICTOR (human) ( 6 ) , RICTOR (mouse) ( 6 ) , Sin1 (mouse) ( 2 , 7 )
Treatments:
BX795 ( 7 ) , cycloheximide ( 5 ) , IGF-1 ( 8 ) , insulin ( 1 , 7 ) , ischemia ( 3 ) , PAN ( 5 ) , PDGF ( 8 ) , PIK90 ( 7 ) , PP242 ( 7 ) , rapamycin ( 6 , 7 ) , serum ( 6 ) , SP600125 ( 3 ) , Torin1 ( 5 )

Downstream Regulation
Effects of modification on Akt1:
ubiquitination ( 5 )
Effects of modification on biological processes:
translation, altered ( 5 )

References 

1

Schrötter S, Leondaritis G, Eickholt BJ (2016) Capillary Isoelectric Focusing of Akt Isoforms Identifies Highly Dynamic Phosphorylation in Neuronal Cells and Brain Tissue. J Biol Chem 291, 10239-51
26945062   Curated Info

2

Warfel NA, Niederst M, Newton AC (2011) Disruption of the interface between the pleckstrin homology (PH) and kinase domains of Akt protein is sufficient for hydrophobic motif site phosphorylation in the absence of mTORC2. J Biol Chem 286, 39122-9
21908613   Curated Info

3

Wei J, et al. (2011) c-Jun N-terminal kinase (JNK-1) confers protection against brief but not extended ischemia during acute myocardial infarction. J Biol Chem 286, 13995-4006
21324895   Curated Info

4

Xie X, et al. (2011) IkappaB kinase epsilon and TANK-binding kinase 1 activate AKT by direct phosphorylation. Proc Natl Acad Sci U S A 108, 6474-9
21464307   Curated Info

5

Oh WJ, et al. (2010) mTORC2 can associate with ribosomes to promote cotranslational phosphorylation and stability of nascent Akt polypeptide. EMBO J 29, 3939-3951
21045808   Curated Info

6

Treins C, et al. (2010) Rictor is a novel target of p70 S6 kinase-1. Oncogene 29, 1003-16
19935711   Curated Info

7

Feldman ME, et al. (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7, e38
19209957   Curated Info

8

Toker A, Newton AC (2000) Akt/protein kinase B is regulated by autophosphorylation at the hypothetical PDK-2 site. J Biol Chem 275, 8271-4
10722653   Curated Info