Tyr315
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Home > Phosphorylation Site Page: > Tyr315  -  Akt1 (mouse)

Site Information
tFCGtPEyLAPEVLE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448519

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 ) , mutation of modification site ( 5 ) , phospho-antibody ( 4 , 5 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 3 ) , 293 (epithelial) ( 5 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 5 ) , brain ( 1 ) , COS (fibroblast) ( 4 , 5 ) , embryo ( 1 ) , liver ( 1 ) , PC3 (prostate cell) ( 5 )

Upstream Regulation
Putative in vivo kinases:
Src (mouse) ( 5 )
Kinases, in vitro:
Src (mouse) ( 5 )
Phosphatases, in vitro:
PTPRB (mouse) ( 5 )
Treatments:
EGF ( 5 ) , LY294002 ( 5 ) , PP2 ( 5 )

Downstream Regulation
Effects of modification on Akt1:
enzymatic activity, induced ( 2 , 5 )
Effects of modification on biological processes:
apoptosis, altered ( 5 )

References 

1

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

2

Zheng Y, et al. (2010) Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Mol Cell Biol 30, 4280-92
20606012   Curated Info

3

Tucker M (2009) CST Curation Set: 8606; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

4

Jiang T, Qiu Y (2003) Interaction between Src and a C-terminal proline-rich motif of Akt is required for Akt activation. J Biol Chem 278, 15789-93
12600984   Curated Info

5

Chen R, et al. (2001) Regulation of Akt/PKB activation by tyrosine phosphorylation. J Biol Chem 276, 31858-62
11445557   Curated Info