Ser222
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.8
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser222  -  MEK1 (rat)

Site Information
LIDsMANsFVGTRSY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448513

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 11 , 12 ) , peptide sequencing ( 12 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 ) , phosphopeptide mapping ( 11 ) , western blotting ( 1 , 2 , 4 , 6 , 7 , 8 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 6 ) , 'neuron, bipolar' ( 8 ) , 'neuron, cerebellar granule' ( 2 ) , COS (fibroblast) ( 7 ) , preovulatory follicle granulosa ( 1 ) , REF52 (fibroblast) ( 4 , 7 ) , VSMC-aorta ( 5 )

Upstream Regulation
Regulatory protein:
PAK1 (rat) ( 7 )
Putative in vivo kinases:
MEK1 (rat) ( 4 )
Kinases, in vitro:
BRAF (rat) ( 4 ) , MEK1 (rat) ( 4 ) , MEKK1 (mouse) ( 10 )
Treatments:
AG879 ( 8 ) , BDNF ( 8 ) , EGF ( 4 , 7 ) , fibronectin ( 4 , 7 ) , NGF ( 11 ) , NSC23766 ( 2 ) , okadaic_acid ( 6 ) , PD98059 ( 8 ) , PP2 ( 7 ) , Ro31-8220 ( 8 ) , tautomycin ( 1 ) , TeTx ( 8 ) , urofollitropin ( 1 ) , vanadate ( 1 ) , wortmannin ( 8 )

Downstream Regulation
Effects of modification on MEK1:
enzymatic activity, induced ( 10 , 11 )

References 

1

Donaubauer EM, Law NC, Hunzicker-Dunn ME (2016) Follicle-Stimulating Hormone (FSH)-dependent Regulation of Extracellular Regulated Kinase (ERK) Phosphorylation by the Mitogen-activated Protein (MAP) Kinase Phosphatase MKP3. J Biol Chem 291, 19701-12
27422819   Curated Info

2

Stankiewicz TR, et al. (2015) Neuronal Apoptosis Induced by Selective Inhibition of Rac GTPase versus Global Suppression of Rho Family GTPases Is Mediated by Alterations in Distinct Mitogen-activated Protein Kinase Signaling Cascades. J Biol Chem 290, 9363-76
25666619   Curated Info

3

Flinder LI, et al. (2011) EGF-induced ERK-activation downstream of FAK requires rac1-NADPH oxidase. J Cell Physiol 226, 2267-78
21660950   Curated Info

4

Park ER, Eblen ST, Catling AD (2007) MEK1 activation by PAK: a novel mechanism. Cell Signal 19, 1488-96
17314031   Curated Info

5

Pinzar E, et al. (2005) Angiotensin II induces tyrosine nitration and activation of ERK1/2 in vascular smooth muscle cells. FEBS Lett 579, 5100-4
16139272   Curated Info

6

Mao L, et al. (2005) Role of protein phosphatase 2A in mGluR5-regulated MEK/ERK phosphorylation in neurons. J Biol Chem 280, 12602-10
15661743   Curated Info

7

Slack-Davis JK, et al. (2003) PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation. J Cell Biol 162, 281-91
12876277   Curated Info

8

Gil C, Chaib-Oukadour I, Aguilera J (2003) C-terminal fragment of tetanus toxin heavy chain activates Akt and MEK/ERK signalling pathways in a Trk receptor-dependent manner in cultured cortical neurons. Biochem J 373, 613-20
12710887   Curated Info

9

Reusch HP, et al. (2001) Regulation of Raf by Akt controls growth and differentiation in vascular smooth muscle cells. J Biol Chem 276, 33630-7
11443134   Curated Info

10

Yan M, Templeton DJ (1994) Identification of 2 serine residues of MEK-1 that are differentially phosphorylated during activation by raf and MEK kinase. J Biol Chem 269, 19067-73
8034665   Curated Info

11

Alessi DR, et al. (1994) Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J 13, 1610-9
8157000   Curated Info

12

Saito Y, et al. (1994) The threonine residues in MAP kinase kinase 1 phosphorylated by MAP kinase in vitro are also phosphorylated in nerve growth factor-stimulated rat phaeochromocytoma (PC12) cells. FEBS Lett 341, 119-24
8137910   Curated Info