Ser36
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Home > Phosphorylation Site Page: > Ser36  -  SHC1 (human)

Site Information
TPPEELPsPSASSLG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448508

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 2 , 4 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 1 , 2 , 3 , 4 )
Disease tissue studied:
breast cancer ( 1 ) , prostate cancer ( 3 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
MKK4 (human) ( 7 )
Phosphatases, in vitro:
PPP2CA (human) ( 7 )
Treatments:
A23187 ( 6 , 8 ) , AG1478 ( 4 ) , anti-CD3 ( 6 , 8 ) , arsenic ( 1 ) , beta-amyloid_40 ( 7 ) , CML ( 4 ) , ebselen ( 7 ) , EGF ( 4 ) , EGTA ( 6 ) , Fas ( 8 ) , Go_6976 ( 2 ) , H2O2 ( 2 , 5 , 6 , 8 ) , methylglyoxal ( 4 ) , PEITC ( 3 ) , phenformin ( 1 ) , SP600125 ( 7 ) , thapsigargin ( 6 ) , U0126 ( 4 ) , UV ( 5 )

Downstream Regulation
Effects of modification on SHC1:
intracellular localization ( 1 ) , molecular association, regulation ( 5 ) , phosphorylation ( 1 , 7 )
Effects of modification on biological processes:
apoptosis, induced ( 6 , 7 , 8 ) , carcinogenesis, induced ( 1 )
Inhibit interaction with:
MIA (human) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 1 )

References 

1

Lewis K, et al. (2020) p66ShcA functions as a contextual promoter of breast cancer metastasis. Breast Cancer Res 22, 7
31941526   Curated Info

2

Haller M, et al. (2016) Novel Insights into the PKCĪ²-dependent Regulation of the Oxidoreductase p66Shc. J Biol Chem 291, 23557-23568
27624939   Curated Info

3

Xiao D, Singh SV (2010) p66Shc is indispensable for phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells. Cancer Res 70, 3150-8
20354186   Curated Info

4

Cai W, et al. (2008) AGE-receptor-1 counteracts cellular oxidant stress induced by AGEs via negative regulation of p66shc-dependent FKHRL1 phosphorylation. Am J Physiol Cell Physiol 294, C145-52
18032526   Curated Info

5

Kasuno K, et al. (2007) Antagonism of p66shc by melanoma inhibitory activity. Cell Death Differ 14, 1414-21
17431427   Curated Info

6

Pellegrini M, et al. (2007) p66SHC promotes T cell apoptosis by inducing mitochondrial dysfunction and impaired Ca2+ homeostasis. Cell Death Differ 14, 338-47
16794602   Curated Info

7

Smith WW, et al. (2005) Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity. J Cell Biol 169, 331-9
15837797   Curated Info

8

Pacini S, et al. (2004) p66SHC promotes apoptosis and antagonizes mitogenic signaling in T cells. Mol Cell Biol 24, 1747-57
14749389   Curated Info