Ser144
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Home > Phosphorylation Site Page: > Ser144  -  PAK1 (mouse)

Site Information
SNSKKYMsFTDKSAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448170

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 18 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 11 , 18 ) , western blotting ( 1 , 2 , 3 , 4 , 11 , 18 )
Disease tissue studied:
leukemia ( 6 ) , acute myelogenous leukemia ( 6 ) , lymphoma ( 2 ) , anaplastic large cell lymphoma ( 2 )
Relevant cell line - cell type - tissue:
'fat, brown' ( 10 ) , 'stem, embryonic' ( 13 , 14 , 15 , 16 , 17 ) , A431 (epithelial) ( 2 ) , AG01522 (fibroblast) ( 2 ) , blood ( 6 ) , brain ( 7 , 8 , 10 , 12 ) , colon ( 1 ) , HaCaT (keratinocyte) ( 2 ) , JB-6 (lymphocyte) ( 2 ) , keratinocyte-skin ( 2 ) , kidney ( 10 ) , liver ( 11 ) , lung ( 10 ) , macrophage-peritoneum ( 5 ) , MEF (fibroblast) ( 9 , 11 ) , neuron ( 3 ) , neutrophil ( 18 ) , skin ( 2 ) , spleen ( 10 ) , T lymphocyte ( 1 ) , T lymphocyte-lymph node ( 4 ) , testis ( 10 )

Upstream Regulation
Regulatory protein:
PKCH (mouse) ( 4 )
Treatments:
fMLP ( 18 ) , UVB ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
colonic inflamation ( 1 )

References 

1

Lyons J, et al. (2018) Integrated in vivo multiomics analysis identifies p21-activated kinase signaling as a driver of colitis. Sci Signal 11
29487189   Curated Info

2

Beesetti S, et al. (2017) Transcriptional regulation of ataxia-telangiectasia and Rad3-related protein by activated p21-activated kinase-1 protects keratinocytes in UV-B-induced premalignant skin lesions. Oncogene 36, 6154-6163
28692051   Curated Info

3

Hu B, et al. (2016) Tuning PAK Activity to Rescue Abnormal Myelin Permeability in HNPP. PLoS Genet 12, e1006290
27583434   Curated Info

4

Kong KF, et al. (2014) Protein kinase C-η controls CTLA-4-mediated regulatory T cell function. Nat Immunol 15, 465-72
24705298   Curated Info

5

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

6

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

7

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

8

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

9

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

10

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

11

Deguchi A, et al. (2010) LKB1 suppresses p21-activated kinase-1 (PAK1) by phosphorylation of Thr109 in the p21-binding domain. J Biol Chem 285, 18283-90
20400510   Curated Info

12

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

13

Tucker M (2009) CST Curation Set: 8601; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

14

Tucker M (2009) CST Curation Set: 8604; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

15

Tucker M (2009) CST Curation Set: 8603; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

16

Tucker M (2009) CST Curation Set: 8607; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

17

Tucker M (2009) CST Curation Set: 8600; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

18

Zhan Q, et al. (2003) p21-activated kinase 2 in neutrophils can be regulated by phosphorylation at multiple sites and by a variety of protein phosphatases. J Immunol 171, 3785-93
14500679   Curated Info

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