Ser153
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Home > Phosphorylation Site Page: > Ser153  -  VASP (mouse)

Site Information
EHMERRVsNAGGPPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448379

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 6 , 7 ) , mass spectrometry ( 4 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 3 , 5 , 6 ) , western blotting ( 1 , 3 , 5 )
Disease tissue studied:
anthrax infection ( 4 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 1 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 ) , aorta ( 1 ) , bladder ( 1 ) , CV1 (fibroblast) [PKG1 iso2 (mouse)] ( 7 ) , CV1 (fibroblast) [VASP (mouse)] ( 7 ) , ECV304 (endothelial) ( 6 ) , lung ( 1 ) , MDCK (epithelial) ( 6 ) , MEF (fibroblast) ( 1 ) , neutrophil-blood ( 3 ) , platelet ( 1 ) , platelet-blood ( 5 ) , REF52 (fibroblast) ( 6 ) , spleen ( 4 ) , VSMC ('muscle, smooth') ( 1 ) , WI-38 (fibroblast) ( 6 )

Upstream Regulation
Regulatory protein:
PKCD (mouse) ( 5 ) , PKG1 (mouse) ( 1 )
Putative in vivo kinases:
PKACA (human) ( 3 ) , PKG1 (human) ( 3 ) , PKG1 iso2 (mouse) ( 7 )
Kinases, in vitro:
PKG1 iso2 (mouse) ( 7 )
Treatments:
calyculin_A ( 1 ) , cAMP_analog ( 1 ) , cell_adhesion ( 6 ) , cell_detachment ( 6 ) , cGMP_analog ( 1 , 3 , 7 ) , colforsin ( 6 ) , collagen ( 5 ) , DEA-NONOate ( 1 ) , fMLP ( 3 ) , IBMX ( 6 ) , siRNA ( 3 )

Downstream Regulation
Effects of modification on VASP:
molecular association, regulation ( 3 , 6 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 3 )
Induce interaction with:
Abl iso4 (mouse) ( 6 )
Inhibit interaction with:
ACTB (mouse) ( 3 )

References 

1

Vallur R, Kalbacher H, Feil R (2014) Catalytic Activity of cGMP-Dependent Protein Kinase Type I in Intact Cells Is Independent of N-Terminal Autophosphorylation. PLoS One 9, e98946
24897423   Curated Info

2

Thomson DM, et al. (2011) Phosphorylation of VASP by AMPK alters actin binding and occurs at a novel site. Biochem Biophys Res Commun 414, 215-9
21945940   Curated Info

3

Thom SR, et al. (2011) Neutrophil beta2 integrin inhibition by enhanced interactions of vasodilator-stimulated phosphoprotein with S-nitrosylated actin. J Biol Chem 286, 32854-65
21795685   Curated Info

4

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

5

Pula G, et al. (2006) PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation. Blood 108, 4035-44
16940418   Curated Info

6

Howe AK, Hogan BP, Juliano RL (2002) Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion. J Biol Chem 277, 38121-6
12087107   Curated Info

7

Collins SP, Uhler MD (1999) Cyclic AMP- and cyclic GMP-dependent protein kinases differ in their regulation of cyclic AMP response element-dependent gene transcription. J Biol Chem 274, 8391-404
10085070   Curated Info