Thr371
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Home > Phosphorylation Site Page: > Thr371  -  TRF1 (human)

Site Information
VSKsQPVtPEKHRAR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3727647
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 9 ) , immunoassay ( 1 , 4 , 9 ) , mass spectrometry ( 3 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 2 , 4 , 9 , 13 ) , phospho-antibody ( 1 , 2 , 4 , 9 ) , western blotting ( 2 , 4 , 9 , 13 )
Disease tissue studied:
bone cancer ( 2 ) , breast cancer ( 3 , 4 ) , breast ductal carcinoma ( 4 ) , breast cancer, triple negative ( 3 , 4 ) , lymphoma ( 1 ) , Hodgkin's lymphoma ( 1 ) , fibrosarcoma of soft tissue ( 4 , 9 )
Relevant cell line - cell type - tissue:
293P (epithelial) ( 2 ) , BJ1-hTERT ( 4 ) , breast ( 3 ) , GM637 ( 4 ) , GM847 (fibroblast) ( 2 ) , HCC1937 (breast cell) ( 4 ) , HDLM-2 (lymphoid) ( 1 ) , HeLa (cervical) ( 4 , 9 , 10 , 13 ) , HeLa S3 (cervical) ( 8 ) , HT1080 (fibroblast) ( 4 , 9 ) , hTERT-RPE1 (epithelial) ( 9 ) , Jurkat (T lymphocyte) ( 6 , 7 , 11 , 12 ) , L1236 (lymphoid) ( 1 ) , L428 (lymphoid) ( 1 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Regulatory protein:
53BP1 (human) ( 4 ) , BRCA1 (human) ( 4 ) , RIF1 (human) ( 4 )
Putative in vivo kinases:
CDK1 (human) ( 4 , 9 )
Kinases, in vitro:
CDK1 (human) ( 9 , 13 )
Treatments:
CGP74514A ( 4 , 9 ) , DMSO ( 9 ) , mirin ( 4 ) , nocodazole ( 9 ) , okadaic_acid ( 9 ) , seliciclib ( 4 , 9 )

Downstream Regulation
Effects of modification on TRF1:
intracellular localization ( 2 ) , molecular association, regulation ( 4 , 9 ) , phosphorylation ( 4 , 13 ) , protein stabilization ( 9 )
Effects of modification on biological processes:
apoptosis, induced ( 9 ) , carcinogenesis, induced ( 1 ) , cell cycle regulation ( 4 , 9 ) , chromatin organization, altered ( 9 ) , DNA repair, induced ( 4 )
Induce interaction with:
53BP1 (human) ( 4 ) , DNA ( 4 ) , H2AX (human) ( 4 )
Inhibit interaction with:
DNA ( 9 )

Disease / Diagnostics Relevance
Relevant diseases:
Hodgkin's lymphoma ( 1 )

References 

1

Lima MF, et al. (2022) Consecutive Inhibition of Telomerase and Alternative Lengthening Pathway Promotes Hodgkin's Lymphoma Cell Death. Biomedicines 10
36140400   Curated Info

2

Wilson FR, Ho A, Walker JR, Zhu XD (2016) Cdk-dependent phosphorylation regulates TRF1 recruitment to PML bodies and promotes C-circle production in ALT cells. J Cell Sci 129, 2559-72
27185864   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

McKerlie M, et al. (2013) Phosphorylated (pT371)TRF1 is recruited to sites of DNA damage to facilitate homologous recombination and checkpoint activation. Nucleic Acids Res 41, 10268-82
23997120   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Guo A (2011) CST Curation Set: 12435; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

7

Guo A (2011) CST Curation Set: 12436; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

8

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

9

McKerlie M, Zhu XD (2011) Cyclin B-dependent kinase 1 regulates human TRF1 to modulate the resolution of sister telomeres. Nat Commun 2, 371
21712819   Curated Info

10

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

11

Possemato A (2009) CST Curation Set: 6922; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

12

Possemato A (2008) CST Curation Set: 5276; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

13

Wu ZQ, Yang X, Weber G, Liu X (2008) Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem 283, 25503-13
18625707   Curated Info

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