Ser3
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Site Information
_____MAsGVAVsDG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448444
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 95 ) , [32P] bio-synthetic labeling ( 11 , 90 , 103 ) , immunoassay ( 29 , 35 ) , immunoprecipitation ( 11 , 13 , 14 , 33 , 70 , 97 ) , mass spectrometry ( 11 , 12 , 15 , 17 , 20 , 24 , 28 , 30 , 31 , 34 , 36 , 38 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 48 , 49 , 50 , 54 , 55 , 57 , 58 , 59 , 60 , 61 , 62 , 64 , 65 , 66 , 67 , 68 , 69 , 71 , 72 , 75 , 76 , 78 , 80 , 81 , 83 , 92 ) , microscopy-colocalization with upstream kinase ( 22 , 79 , 97 ) , mutation of modification site ( 6 , 12 , 19 , 21 , 22 , 23 , 26 , 27 , 39 , 63 , 73 , 77 , 79 , 82 , 85 , 95 , 101 , 103 , 104 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 14 , 16 , 19 , 21 , 22 , 23 , 25 , 27 , 29 , 32 , 33 , 35 , 37 , 39 , 51 , 52 , 53 , 56 , 63 , 70 , 73 , 74 , 77 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 104 ) , western blotting ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 16 , 19 , 21 , 22 , 23 , 27 , 29 , 32 , 33 , 35 , 37 , 39 , 51 , 52 , 56 , 63 , 70 , 73 , 74 , 77 , 79 , 84 , 86 , 87 , 89 , 90 , 91 , 97 , 99 , 104 )
Disease tissue studied:
adrenal cancer ( 16 ) , pheochromocytoma ( 16 ) , bone cancer ( 26 ) , breast cancer ( 4 , 6 , 9 , 30 , 33 , 39 , 42 , 63 , 77 , 85 ) , breast adenocarcinoma ( 9 , 33 ) , breast ductal carcinoma ( 30 ) , HER2 positive breast cancer ( 17 ) , luminal A breast cancer ( 17 ) , luminal B breast cancer ( 17 ) , breast cancer, surrounding tissue ( 17 ) , breast cancer, triple negative ( 6 , 9 , 17 , 30 , 33 ) , cervical cancer ( 65 ) , cervical adenocarcinoma ( 65 ) , colorectal cancer ( 11 , 52 ) , colorectal carcinoma ( 11 , 52 ) , leukemia ( 5 , 8 , 32 , 43 , 48 , 50 , 84 ) , acute myelogenous leukemia ( 43 , 48 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 32 , 41 , 43 ) , acute megakaryoblastic leukemia (M7) ( 41 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 41 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 41 ) , acute myeloblastic leukemia, without maturation (M1) ( 41 ) , chronic myelogenous leukemia ( 32 ) , T cell leukemia ( 8 , 32 , 50 ) , lung cancer ( 34 , 42 , 57 ) , non-small cell lung cancer ( 42 ) , small-cell lung cancer ( 34 ) , lymphoma ( 32 ) , B cell lymphoma ( 41 ) , non-Hodgkin's lymphoma ( 41 ) , T cell lymphoma ( 32 ) , neuroblastoma ( 16 , 51 , 95 ) , ovarian cancer ( 7 , 11 , 30 ) , multiple myeloma ( 41 ) , prostate cancer ( 63 ) , melanoma skin cancer ( 24 ) , cancer, squamous cell carcinoma ( 21 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 49 ) , 'muscle, smooth' [Cofilin-1 (human), transfection] ( 73 ) , 'neuron, dorsal root ganglion'-brain ( 99 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 59 ) , 293 (epithelial) ( 12 , 14 , 16 , 26 , 69 ) , 293GP (epithelial) [NPM-ALK (human), transfection] ( 58 ) , 3T3 (fibroblast) ( 104 ) , 786-O (renal) [VHL (human), transfection] ( 20 ) , 786-O (renal) ( 20 ) , A498 (renal) ( 62 ) , A549 (pulmonary) ( 36 ) , ACT-1 (thyroid cell) ( 8 ) , AML-193 (monocyte) ( 41 ) , bEnd3 ( 13 ) , BHK-21 (fibroblast) ( 97 ) , breast ( 17 , 30 ) , BT-20 (breast cell) ( 42 ) , BT-549 (breast cell) ( 6 , 9 , 42 ) , Calu 6 (pulmonary) ( 42 ) , Caov-3 (ovarian) ( 7 ) , Chang liver (cervical) ( 78 ) , CHO-K1 (fibroblast) ( 22 ) , CL1-0 (pulmonary) ( 57 ) , CL1-1 (pulmonary) ( 57 ) , CL1-2 (pulmonary) ( 57 ) , CL1-5 (pulmonary) ( 57 ) , CMK (megakaryoblast) ( 41 ) , CML-T1 (T lymphocyte) ( 32 ) , COLO-205 (intestinal) ( 11 ) , COS (fibroblast) ( 77 , 83 , 90 , 93 , 97 , 99 , 103 ) , CTS (myeloid) ( 41 ) , CX-1 (intestinal) ( 11 ) , DMS79 (pulmonary) ( 34 ) , DOHH2 ('B lymphocyte, precursor') ( 41 ) , endothelial-aorta ( 44 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 46 ) , Flp-In T-Rex-293 (epithelial) ( 46 ) , GM00130 (B lymphocyte) ( 61 ) , H2009 (pulmonary) ( 42 ) , H2077 (pulmonary) ( 42 ) , H2887 (pulmonary) ( 42 ) , H322M (pulmonary) ( 42 ) , HaCaT (keratinocyte) ( 21 ) , HCC1359 (pulmonary) ( 42 ) , HCC1937 (breast cell) ( 42 ) , HCC2279 (pulmonary) ( 42 ) , HCC366 (pulmonary) ( 42 ) , HCC4006 (pulmonary) ( 42 ) , HCC78 (pulmonary) ( 42 ) , HCC827 (pulmonary) ( 42 ) , HCT116 (intestinal) ( 11 , 52 , 68 ) , HEK293T (epithelial) ( 19 , 87 , 91 , 93 , 94 ) , HEL (erythroid) ( 32 , 41 , 43 ) , HeLa (cervical) [LIMK2 iso2 (human), transfection] ( 63 ) , HeLa (cervical) ( 10 , 14 , 15 , 26 , 28 , 33 , 35 , 37 , 40 , 52 , 53 , 56 , 66 , 70 , 75 , 79 , 91 , 96 , 100 , 101 ) , HeLa S3 (cervical) ( 54 , 65 ) , HeLa_Meta (cervical) ( 60 ) , HeLa_Pro (cervical) ( 60 ) , HeLa_Telo (cervical) ( 60 ) , HL60 (myeloid) ( 5 , 32 , 84 ) , HN5 (squamous) ( 21 ) , HOP62 (pulmonary) ( 42 ) , HUES-7 ('stem, embryonic') ( 67 ) , HUES-9 ('stem, embryonic') ( 55 ) , HUVEC (endothelial) ( 88 ) , Jurkat (T lymphocyte) ( 8 , 32 , 38 , 72 , 80 , 81 , 98 ) , JURL-MK1 ( 32 ) , K562 (erythroid) ( 32 , 40 , 66 ) , Karpas-299 (T lymphocyte) ( 32 ) , Kasumi-1 (myeloid) ( 41 ) , KG-1 (myeloid) ( 41 , 48 ) , Kit225 (T lymphocyte) ( 50 ) , LCLC-103H (pulmonary) ( 42 ) , leukocyte-blood ( 64 ) , LNCaP (prostate cell) ( 63 ) , LOU-NH91 (squamous) ( 42 ) , LS180 (intestinal) ( 11 ) , lung ( 34 ) , MCF-10A (breast cell) ( 39 ) , MCF-7 (breast cell) ( 4 , 42 , 63 , 77 , 93 , 94 ) , MDA-MB-231 (breast cell) ( 6 , 9 , 42 ) , MDA-MB-435S (breast cell) ( 68 ) , MDA-MB-468 (breast cell) ( 9 , 33 , 42 ) , MDCK (epithelial) ( 82 ) , mesangial ( 86 ) , MOLM-7 (myeloid) ( 32 ) , MSS31 (endothelial) ( 88 ) , MTE4-14 ( 3 ) , MTLn3 (breast cell) ( 85 ) , MV4-11 (macrophage) ( 41 , 43 , 68 ) , NCI-H128 (pulmonary) ( 34 ) , NCI-H1395 (pulmonary) ( 42 ) , NCI-H1417 (pulmonary) ( 34 ) , NCI-H1568 (pulmonary) ( 42 ) , NCI-H157 (pulmonary) ( 42 ) , NCI-H1648 (pulmonary) ( 42 ) , NCI-H1666 (pulmonary) ( 42 ) , NCI-H2030 (pulmonary) ( 42 ) , NCI-H209 (pulmonary) ( 34 ) , NCI-H2172 (pulmonary) ( 42 ) , NCI-H322 (pulmonary) ( 42 ) , NCI-H460 (pulmonary) ( 42 ) , NCI-H520 (squamous) ( 42 ) , NCI-H524 (pulmonary) ( 34 ) , NCI-H647 (pulmonary) ( 42 ) , neuron-'brain, hippocampus' ( 22 , 23 ) , neutrophil-blood ( 12 ) , OPM-2 (plasma cell) ( 41 ) , OSE (epithelial) ( 92 ) , outer hair cells (OHC) ( 25 ) , ovary ( 30 ) , OVCAR3 (ovarian) ( 7 ) , P31/FUJ (erythroid) ( 41 , 43 ) , PC-12 (chromaffin) ( 16 ) , PC9 (pulmonary) ( 42 ) , PEER ( 52 ) , platelet-blood ( 89 ) , RL ('B lymphocyte, precursor') ( 41 ) , RPMI-8266 (plasma cell) ( 41 ) , Schwann ( 29 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 31 ) , SH-SY5Y (neural crest) ( 16 , 31 , 95 ) , SHEP (neuron) ( 51 ) , SKOV-3 (ovarian) ( 7 ) , SU-DHL-6 (B lymphocyte) ( 41 ) , T lymphocyte-blood ( 45 , 74 , 98 ) , TERT20 ('stem, mesenchymal') ( 76 ) , U266 (plasma cell) ( 41 ) , U2OS (bone cell) ( 26 ) , VACO432 (ovarian) ( 11 ) , WM115 (melanocyte) ( 71 ) , WM239A (melanocyte) ( 24 )

Upstream Regulation
Regulatory protein:
ACTG1 (human) ( 51 ) , ARHGEF7 (human) ( 7 ) , BAG1 (human) ( 4 ) , CAP1 (human) ( 10 , 37 ) , CDK18 (human) ( 14 ) , galectin-4 (human) ( 11 ) , HSP70 (human) ( 52 ) , ILK (human) ( 7 ) , LATS1 (human) ( 9 ) , LIMK1 (human) ( 77 ) , LIMK2 iso2 (human) ( 63 ) , Merlin (mouse) ( 29 ) , MYO6 (human) ( 16 ) , NISCH (human) ( 77 ) , NISCH (mouse) ( 77 ) , PAK4 (human) ( 35 ) , PDPN (human) ( 21 ) , PKD2 (human) ( 33 ) , PLCG1 (rat) ( 85 ) , PPIL5 (human) ( 56 ) , PRKD3 (human) ( 33 ) , PTEN (human) ( 39 ) , RAC3 (human) ( 7 ) , RASA4 (human) ( 5 ) , RHOC (human) ( 21 ) , ROCK1 (human) ( 27 , 103 ) , SPRY4 (human) ( 90 ) , SSH1 (human) ( 70 ) , TACSTD2 (human) ( 3 ) , TESK1 (human) ( 90 ) , YAP1 (human) ( 9 )
Putative in vivo kinases:
LIMK1 (human) ( 79 , 82 , 85 , 88 , 97 , 99 ) , LIMK2 (human) ( 103 ) , PRKD1 (human) ( 70 ) , ROCK2 (mouse) ( 22 ) , TESK1 (rat) ( 90 , 100 )
Kinases, in vitro:
LIMK1 (human) ( 19 , 74 , 88 , 98 , 99 , 101 , 102 ) , LIMK2 (human) ( 101 ) , TESK1 (rat) ( 100 )
Putative upstream phosphatases:
PPP3CA (human) ( 91 ) , SSH1 (human) ( 91 , 94 , 96 )
Phosphatases, in vitro:
PPP3CA (human) ( 91 ) , SSH1 (human) ( 91 , 93 , 96 )
Treatments:
8TQ ( 8 ) , A23187 ( 91 ) , ACh ( 73 ) , angiotensin-(1-7) ( 44 ) , antibody ( 45 , 72 ) , Auranofin ( 8 ) , b-AP15 ( 8 ) , bisindolylmaleimide ( 25 ) , Ca(2+) ( 73 , 91 ) , catalase ( 74 ) , cell_detachment ( 39 ) , cepharanthine ( 6 ) , ciclosporin ( 73 ) , CK-666 ( 8 ) , CTGF ( 86 ) , CXCL12 ( 98 ) , delta-methrin ( 73 ) , depolarization ( 73 ) , EGF ( 15 ) , endothelin ( 7 ) , epirubicin ( 6 ) , erucin ( 27 ) , fibronectin ( 100 ) , FK506 ( 91 ) , fluid_shear_stress ( 12 ) , fMLP ( 5 , 74 ) , GNF2 ( 12 ) , H1152 ( 89 ) , H2O2 ( 74 ) , HBX 41,108 ( 8 ) , heat_shock ( 52 ) , histamine ( 91 ) , IL-8 ( 84 ) , imatinib ( 12 ) , insulin ( 94 ) , interleukin-6-hydroxymethyl-chiro-inositol-2(R)-2-methyl-3-O-octadecylcarbonate ( 86 ) , ionizing_radiation ( 63 ) , IPA-3 ( 32 ) , ischemia ( 30 ) , laminin-1 ( 90 ) , latrunculin_B ( 8 ) , LPA ( 25 , 103 ) , LY294002 ( 84 , 86 , 94 ) , metastatic potential ( 57 ) , MG132 ( 60 ) , MG132_withdrawal ( 60 ) , NAC ( 8 ) , neuregulin ( 93 ) , nocodazole ( 63 , 65 ) , okadaic_acid ( 91 ) , peptide inhibitor ( 84 ) , phorbol_ester ( 25 ) , PP2 ( 13 ) , PR-619 ( 8 ) , radicicol ( 87 ) , RGDS ( 89 ) , SB203580 ( 88 ) , Sem3A ( 99 ) , serum ( 77 ) , siRNA ( 9 , 70 , 77 ) , staurosporine ( 95 ) , tanespimycin ( 87 ) , thrombin ( 89 ) , VEGF ( 88 ) , vemurafenib ( 24 ) , VLX1570 ( 8 ) , wortmannin ( 84 , 94 ) , WP1130 ( 8 ) , Y27632 ( 13 , 27 , 89 , 100 , 103 )

Downstream Regulation
Effects of modification on Cofilin-1:
activity, induced ( 29 , 85 ) , activity, inhibited ( 7 , 10 , 73 , 74 ) , intracellular localization ( 6 , 27 , 35 , 53 , 79 , 94 , 95 , 104 ) , molecular association, regulation ( 37 , 97 ) , phosphorylation ( 97 )
Effects of modification on biological processes:
apoptosis, inhibited ( 6 , 27 , 32 , 95 ) , carcinogenesis, induced ( 3 , 4 , 7 , 8 ) , cell adhesion, altered ( 101 ) , cell adhesion, induced ( 7 , 22 , 32 ) , cell cycle regulation ( 63 , 79 ) , cell growth, induced ( 3 ) , cell growth, inhibited ( 19 ) , cell motility, altered ( 70 , 74 , 82 , 85 ) , cell motility, induced ( 5 , 7 , 8 , 12 ) , cell motility, inhibited ( 52 ) , cytoskeletal reorganization ( 3 , 4 , 5 , 7 , 8 , 10 , 26 , 29 , 70 , 73 , 74 , 79 , 82 , 97 , 98 , 99 , 101 ) , signaling pathway regulation ( 29 )
Induce interaction with:
14-3-3 zeta (human) ( 97 )
Inhibit interaction with:
CAP1 (human) ( 37 )

Disease / Diagnostics Relevance
Relevant diseases:
Schwannoma ( 29 )

References 

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