Ser219
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Home > Phosphorylation Site Page: > Ser219  -  ATF-4 (human)

Site Information
DTPsDNDsGICMsPE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448436

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 1 ) , immunoprecipitation ( 1 , 3 ) , mutation of modification site ( 1 , 3 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 3 )

Upstream Regulation
Regulatory protein:
RPL41 (human) ( 1 )
Treatments:
colforsin ( 3 ) , IBMX ( 3 )

Downstream Regulation
Effects of modification on ATF-4:
molecular association, regulation ( 2 , 3 ) , protein degradation ( 1 ) , protein stabilization ( 3 )
Effects of modification on biological processes:
transcription, altered ( 3 )
Induce interaction with:
BRD8 iso2 (human) ( 3 ) , FBW1B (human) ( 2 )

References 

1

Wang A, et al. (2011) Ribosomal protein RPL41 induces rapid degradation of ATF4, a transcription factor critical for tumour cell survival in stress. J Pathol 225, 285-92
21706477   Curated Info

2

Pons J, et al. (2008) Transfer-NMR and Docking Studies Identify the Binding of the Peptide Derived from Activating Transcription Factor 4 to Protein Ubiquitin Ligase beta-TrCP. Competition STD-NMR with beta-Catenin. Biochemistry 47, 14-29
18052253   Curated Info

3

Lassot I, et al. (2001) ATF4 degradation relies on a phosphorylation-dependent interaction with the SCF(betaTrCP) ubiquitin ligase. Mol Cell Biol 21, 2192-202
11238952   Curated Info

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