Tyr243
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Home > Phosphorylation Site Page: > Tyr243  -  PDHK1 (human)

Site Information
ARRLCDLyyINSPEL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 462384
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 3 , 4 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 ) , phosphoamino acid analysis ( 1 ) , western blotting ( 1 , 2 )
Disease tissue studied:
brain cancer ( 1 ) , glioblastoma ( 1 ) , glioma ( 1 ) , breast cancer ( 2 ) , leukemia ( 2 ) , acute myelogenous leukemia ( 2 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 2 ) , chronic myelogenous leukemia ( 2 ) , lung cancer ( 2 , 4 ) , non-small cell lung cancer ( 2 ) , small-cell lung cancer ( 4 ) , prostate cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
FGFR1 (human) ( 2 )
Kinases, in vitro:
Abl (human) ( 2 ) , FGFR1 (human) ( 2 ) , FLT3 (human) ( 2 ) , JAK2 (human) ( 2 )

Downstream Regulation
Effects of modification on PDHK1:
intracellular localization ( 2 )

References 

1

Li X, et al. (2016) Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. Mol Cell 61, 705-19
26942675   Curated Info

2

Hitosugi T, et al. (2011) Tyrosine phosphorylation of mitochondrial pyruvate dehydrogenase kinase 1 is important for cancer metabolism. Mol Cell 44, 864-77
22195962   Curated Info

3

Possemato A (2009) CST Curation Set: 8804; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Possemato A (2006) CST Curation Set: 1242; Year: 2006; Biosample/Treatment: cell line, NCI-H82/0.5% serum; Disease: small-cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info