Thr256
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.1.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr256  -  SGK1 (human)

Site Information
EHNSTTStFCGTPEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448431

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 10 ) , mutation of modification site ( 5 , 6 , 7 , 10 ) , phospho-antibody ( 2 , 3 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 9 )
Disease tissue studied:
breast cancer ( 5 , 6 ) , neuroblastoma ( 2 ) , pancreatic cancer ( 3 ) , pancreatic carcinoma ( 3 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 2 ) , 293 (epithelial) ( 7 ) , CFPAC-1 (pancreatic) ( 3 ) , COS (fibroblast) ( 10 ) , KMCH-1 (hepatic) ( 8 ) , MCF-7 (breast cell) ( 6 ) , MDA-MB-231 (breast cell) ( 5 ) , MDCK (epithelial) ( 9 ) , mesangial-kidney ( 4 ) , MzChA (hepatic) ( 8 ) , SH-SY5Y (neural crest) ( 2 ) , SKBr3 (breast cell) ( 5 ) , WM35 (melanocyte) ( 6 )

Upstream Regulation
Regulatory protein:
HSP90A (human) ( 5 , 7 ) , PDK1 (human) ( 7 ) , Raptor (human) ( 6 ) , TSC2 (human) ( 6 )
Putative in vivo kinases:
PDK1 (human) ( 3 ) , PIK3C2A (human) ( 4 )
Kinases, in vitro:
PDK1 (human) ( 3 , 11 )
Treatments:
aldosterone ( 4 ) , amino_acids ( 6 ) , ceramide ( 3 ) , dexamethasone ( 2 ) , EMD638683 ( 2 ) , eplerenone ( 4 ) , estradiol ( 6 ) , geldanamycin ( 5 ) , high-fat diet ( 2 ) , high_glucose ( 2 ) , IL-6 ( 8 ) , insulin ( 6 ) , LY294002 ( 4 , 8 ) , okadaic_acid ( 10 ) , palmitate ( 2 ) , PD98059 ( 4 ) , rapamycin ( 6 ) , SB202190 ( 8 ) , SB203580 ( 4 , 8 ) , siRNA ( 6 ) , streptozotocin ( 2 ) , vanadate ( 7 ) , wortmannin ( 4 , 8 )

Downstream Regulation
Effects of modification on SGK1:
enzymatic activity, induced ( 2 , 3 , 4 , 5 , 9 , 10 , 11 ) , molecular association, regulation ( 3 ) , phosphorylation ( 2 , 3 , 4 )
Effects of modification on biological processes:
apoptosis, inhibited ( 9 ) , signaling pathway regulation ( 4 ) , transcription, induced ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 2 )

References 

1

Gu W, Zheng H, Canessa CM (2023) Phosphatases maintain low catalytic activity of SGK1: DNA damage resets the balance in favor of phosphorylation. J Biol Chem 299, 104941
37343701   Curated Info

2

Elahi M, et al. (2021) High-fat diet-induced activation of SGK1 promotes Alzheimer's disease-associated tau pathology. Hum Mol Genet 30
33890983   Curated Info

3

Caohuy H, et al. (2014) Activation of 3-Phosphoinositide-dependent Kinase 1 (PDK1) and Serum- and Glucocorticoid-induced Protein Kinase 1 (SGK1) by Short-chain Sphingolipid C4-ceramide Rescues the Trafficking Defect of ΔF508-Cystic Fibrosis Transmembrane Conductance Regulator (ΔF508-CFTR). J Biol Chem 289, 35953-68
25384981   Curated Info

4

Terada Y, et al. (2012) Aldosterone stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum- and glucocorticoid-inducible protein kinase-1. Clin Exp Nephrol 16, 81-8
22042038   Curated Info

5

Belova L, et al. (2008) Hsp90 regulates the phosphorylation and activity of serum- and glucocorticoid-regulated kinase-1. J Biol Chem 283, 18821-31
18456663   Curated Info

6

Hong F, et al. (2008) mTOR-raptor binds and activates SGK1 to regulate p27 phosphorylation. Mol Cell 30, 701-11
18570873   Curated Info

7

Yang KJ, et al. (2008) Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding. J Biol Chem 283, 1480-91
18024423   Curated Info

8

Meng F, et al. (2005) IL-6 activates serum and glucocorticoid kinase via p38alpha mitogen-activated protein kinase pathway. Am J Physiol Cell Physiol 289, C971-81
15917303   Curated Info

9

Shelly C, Herrera R (2002) Activation of SGK1 by HGF, Rac1 and integrin-mediated cell adhesion in MDCK cells: PI-3K-dependent and -independent pathways. J Cell Sci 115, 1985-93
11956329   Curated Info

10

Perrotti N, et al. (2001) Activation of serum- and glucocorticoid-induced protein kinase (Sgk) by cyclic AMP and insulin. J Biol Chem 276, 9406-12
11096081   Curated Info

11

Kobayashi T, Cohen P (1999) Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem J 339 ( Pt 2), 319-28
10191262   Curated Info

12

Jones N, Dumont DJ (1998) The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene 17, 1097-108
9764820   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.