Thr256
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Home > Phosphorylation Site Page: > Thr256  -  SGK1 (human)

Site Information
EHNSTTStFCGTPEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448431

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , immunoprecipitation ( 8 ) , mutation of modification site ( 3 , 4 , 5 , 8 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 7 )
Disease tissue studied:
breast cancer ( 3 , 4 ) , pancreatic cancer ( 1 ) , pancreatic carcinoma ( 1 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
HSP90A (human) ( 3 , 5 ) , PDK1 (human) ( 5 ) , Raptor (human) ( 4 ) , TSC2 (human) ( 4 )
Putative in vivo kinases:
PDK1 (human) ( 1 ) , PIK3C2A (human) ( 2 )
Kinases, in vitro:
PDK1 (human) ( 1 , 9 )
Treatments:
aldosterone ( 2 ) , amino_acids ( 4 ) , ceramide ( 1 ) , eplerenone ( 2 ) , estradiol ( 4 ) , geldanamycin ( 3 ) , IL-6 ( 6 ) , insulin ( 4 ) , LY294002 ( 2 , 6 ) , okadaic_acid ( 8 ) , PD98059 ( 2 ) , rapamycin ( 4 ) , SB202190 ( 6 ) , SB203580 ( 2 , 6 ) , siRNA ( 4 ) , vanadate ( 5 ) , wortmannin ( 2 , 6 )

Downstream Regulation
Effects of modification on SGK1:
enzymatic activity, induced ( 1 , 2 , 3 , 7 , 8 , 9 ) , molecular association, regulation ( 1 ) , phosphorylation ( 1 , 2 )
Effects of modification on biological processes:
apoptosis, inhibited ( 7 ) , signaling pathway regulation ( 2 ) , transcription, induced ( 2 )

References 

1

Caohuy H, et al. (2014) Activation of 3-Phosphoinositide-dependent Kinase 1 (PDK1) and Serum- and Glucocorticoid-induced Protein Kinase 1 (SGK1) by Short-chain Sphingolipid C4-ceramide Rescues the Trafficking Defect of ΔF508-Cystic Fibrosis Transmembrane Conductance Regulator (ΔF508-CFTR). J Biol Chem 289, 35953-68
25384981   Curated Info

2

Terada Y, et al. (2012) Aldosterone stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum- and glucocorticoid-inducible protein kinase-1. Clin Exp Nephrol 16, 81-8
22042038   Curated Info

3

Belova L, et al. (2008) Hsp90 regulates the phosphorylation and activity of serum- and glucocorticoid-regulated kinase-1. J Biol Chem 283, 18821-31
18456663   Curated Info

4

Hong F, et al. (2008) mTOR-raptor binds and activates SGK1 to regulate p27 phosphorylation. Mol Cell 30, 701-11
18570873   Curated Info

5

Yang KJ, et al. (2008) Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding. J Biol Chem 283, 1480-91
18024423   Curated Info

6

Meng F, et al. (2005) IL-6 activates serum and glucocorticoid kinase via p38alpha mitogen-activated protein kinase pathway. Am J Physiol Cell Physiol 289, C971-81
15917303   Curated Info

7

Shelly C, Herrera R (2002) Activation of SGK1 by HGF, Rac1 and integrin-mediated cell adhesion in MDCK cells: PI-3K-dependent and -independent pathways. J Cell Sci 115, 1985-93
11956329   Curated Info

8

Perrotti N, et al. (2001) Activation of serum- and glucocorticoid-induced protein kinase (Sgk) by cyclic AMP and insulin. J Biol Chem 276, 9406-12
11096081   Curated Info

9

Kobayashi T, Cohen P (1999) Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem J 339 ( Pt 2), 319-28
10191262   Curated Info

10

Jones N, Dumont DJ (1998) The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene 17, 1097-108
9764820   Curated Info