Ser24
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser24  -  TNNI3 (mouse)

Site Information
APVRRRssANyRAYA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448424
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 26 ) , mass spectrometry ( 6 , 9 , 14 , 15 , 16 , 17 , 18 , 19 , 21 , 22 ) , mutation of modification site ( 2 , 11 , 20 , 26 , 28 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 10 , 13 , 20 , 24 , 28 ) , western blotting ( 1 , 3 , 4 , 5 , 10 , 13 , 20 , 28 )
Disease tissue studied:
melanoma skin cancer ( 14 )
Relevant cell line - cell type - tissue:
'muscle, smooth'-'heart, artery' [TNNI3 (mouse), transgenic] ( 11 ) , cardiac ( 26 ) , heart ( 1 , 2 , 3 , 6 , 9 , 15 , 16 , 17 , 18 , 19 , 21 , 22 , 28 ) , myocardium ( 10 ) , myocyte-heart ( 4 , 5 , 24 ) , myocyte-heart [PMCA4 iso2 (human), transgenic] ( 13 ) , myocyte-heart [TNNI1 (mouse), homozygous knockout] ( 28 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 14 )

Upstream Regulation
Regulatory protein:
ADRB1 (mouse) ( 6 ) , AR (human) ( 4 ) , PKACA (mouse) ( 1 ) , PMCA4 iso2 (human) ( 13 ) , TNNT2 (mouse) ( 10 )
Putative in vivo kinases:
p90RSK (mouse) ( 24 ) , PKACA (mouse) ( 20 ) , ROCK2 (human) ( 25 )
Kinases, in vitro:
p90RSK (mouse) ( 24 ) , PKACA (cow) ( 30 ) , PKACA (human) ( 12 , 29 ) , PKACA (mouse) ( 26 , 28 ) , PKCA (mouse) ( 30 ) , PKCB (mouse) ( 27 ) , PKCB iso2 (mouse) ( 23 ) , PKCE (mouse) ( 27 ) , PRKD1 (human) ( 12 )
Treatments:
H2O2 ( 24 ) , isoproterenol ( 5 , 13 ) , LY333531 ( 24 ) , phenylephrine ( 4 ) , PKCbeta_C2-4_inhibitor ( 24 )

Downstream Regulation
Effects of modification on TNNI3:
activity, induced ( 11 , 28 ) , activity, inhibited ( 20 , 23 , 29 ) , phosphorylation ( 25 ) , protein conformation ( 26 , 28 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 20 , 25 , 28 )

References 

1

Yang L, et al. (2016) Loss of β-adrenergic-stimulated phosphorylation of CaV1.2 channels on Ser1700 leads to heart failure. Proc Natl Acad Sci U S A 113, E7976-E7985
27864509   Curated Info

2

Gresham KS, Stelzer JE (2016) The contributions of cardiac myosin binding protein C and troponin I phosphorylation to β-adrenergic enhancement of in vivo cardiac function. J Physiol 594, 669-86
26635197   Curated Info

3

Yuan CC, et al. (2015) Constitutive phosphorylation of cardiac myosin regulatory light chain prevents development of hypertrophic cardiomyopathy in mice. Proc Natl Acad Sci U S A 112, E4138-46
26124132   Curated Info

4

Wu SC, et al. (2014) Nuclear localization of a1A-adrenergic receptors is required for signaling in cardiac myocytes: an “inside-out” a1-AR signaling pathway. J Am Heart Assoc 3, e000145
24772522   Curated Info

5

Besser J, et al. (2014) MiRNA-1/133a Clusters Regulate Adrenergic Control of Cardiac Repolarization. PLoS One 9, e113449
25415383   Curated Info

6

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

7

Schulz EM, et al. (2012) Tropomyosin dephosphorylation results in compensated cardiac hypertrophy. J Biol Chem 287, 44478-89
23148217   Curated Info

8

Hinken AC, et al. (2012) Protein kinase C depresses cardiac myocyte power output and attenuates myofilament responses induced by protein kinase A. J Muscle Res Cell Motil 33, 439-48
22527640   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Sfichi-Duke L, et al. (2010) Cardiomyopathy-causing deletion K210 in cardiac troponin T alters phosphorylation propensity of sarcomeric proteins. J Mol Cell Cardiol 48, 934-42
20079745   Curated Info

11

Ramirez-Correa GA, et al. (2010) Calcium sensitivity, force frequency relationship and cardiac troponin I: critical role of PKA and PKC phosphorylation sites. J Mol Cell Cardiol 48, 943-53
20083117   Curated Info

12

Bardswell SC, et al. (2010) Distinct sarcomeric substrates are responsible for protein kinase D-mediated regulation of cardiac myofilament Ca2+ sensitivity and cross-bridge cycling. J Biol Chem 285, 5674-82
20018870   Curated Info

13

Mohamed TM, et al. (2009) Specific role of neuronal nitric-oxide synthase when tethered to the plasma membrane calcium pump in regulating the beta-adrenergic signal in the myocardium. J Biol Chem 284, 12091-8
19278978   Curated Info

14

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

15

Possemato A (2008) CST Curation Set: 3846; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

16

Possemato A (2008) CST Curation Set: 3848; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

17

Possemato A (2008) CST Curation Set: 3820; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

18

Possemato A (2008) CST Curation Set: 3830; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

19

Possemato A (2008) CST Curation Set: 3831; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

20

Yasuda S, et al. (2007) Cardiac transgenic and gene transfer strategies converge to support an important role for troponin I in regulating relaxation in cardiac myocytes. Circ Res 101, 377-86
17615373   Curated Info

21

Guo A (2007) CST Curation Set: 3033; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

22

Guo A (2007) CST Curation Set: 3034; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

23

Wang H, et al. (2006) PKC-betaII sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144. J Mol Cell Cardiol 41, 823-33
17010989   Curated Info

24

Itoh S, et al. (2005) Role of p90 ribosomal S6 kinase (p90RSK) in reactive oxygen species and protein kinase C beta (PKC-beta)-mediated cardiac troponin I phosphorylation. J Biol Chem 280, 24135-42
15840586   Curated Info

25

Vahebi S, et al. (2005) Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin. Circ Res 96, 740-7
15774859   Curated Info

26

Sakthivel S, et al. (2005) In vivo and in vitro analysis of cardiac troponin I phosphorylation. J Biol Chem 280, 703-14
15507454   Curated Info

27

Kobayashi T, et al. (2005) A non-equilibrium isoelectric focusing method to determine states of phosphorylation of cardiac troponin I: identification of Ser-23 and Ser-24 as significant sites of phosphorylation by protein kinase C. J Mol Cell Cardiol 38, 213-8
15623438   Curated Info

28

Pi Y, et al. (2003) Protein kinase C and A sites on troponin I regulate myofilament Ca2+ sensitivity and ATPase activity in the mouse myocardium. J Physiol 552, 845-57
12923217   Curated Info

29

Zhang R, Zhao J, Potter JD (1995) Phosphorylation of both serine residues in cardiac troponin I is required to decrease the Ca2+ affinity of cardiac troponin C. J Biol Chem 270, 30773-80
8530519   Curated Info

30

Noland TA, et al. (1995) Cardiac troponin I mutants. Phosphorylation by protein kinases C and A and regulation of Ca(2+)-stimulated MgATPase of reconstituted actomyosin S-1. J Biol Chem 270, 25445-54
7592712   Curated Info